Centro de Investigação Em Química da Universidade Do Porto, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade Do Porto, Porto, Portugal; BioMark/ISEP, Instituto Superior de Engenharia Do Porto, Porto, Portugal.
Centro de Investigação Em Química da Universidade Do Porto, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade Do Porto, Porto, Portugal.
Anal Chim Acta. 2019 Nov 15;1082:126-135. doi: 10.1016/j.aca.2019.07.050. Epub 2019 Jul 26.
Considering the high incidence level and mortality rate of ovarian cancer, particularly among the European female population, the carbohydrate antigen 125 (CA-125) was selected as the protein target for this study for the development of a MIP-based biosensor. This work presents the development of molecular imprinting polymers (MIPs) on gold electrode surface for CA-125 biomarker recognition. The preparation of the CA-125 imprinting was obtained by electropolymerization of pyrrole (Py) monomer in a gold electrode using cyclic voltammetry (CV) in order to obtain highly selective materials with great molecular recognition capability. The quantification of CA-125 biomarker was made through the comparison of two methods: electrochemical (square wave voltammetry -SWV) and optical transduction (surface plasmon resonance -SPR). SWV has been widely used in biological molecules analysis since it is a fast and sensitive technique. In turn, SPR is a non-destructive optical technique that provides high-quality analytical data of CA-125 biomarker interactions with MIP. Several analytical parameters, such as sensitivity, linear response interval, and detection limit were determined to proceed to the performance evaluation of the electrochemical and optical transduction used in the development of the CA-125 biosensor. The biosensor based in the electrochemical transduction was the one that presented the best analytical parameters, yielding a good selectivity and a detection limit (LOD) of 0.01 U/mL, providing a linear concentration range between 0.01 and 500 U/mL. This electrochemical biosensor was selected for the study and it was successfully applied in the CA-125 analysis in artificial serum samples with recovery rates ranging from 91 to 105% with an average relative error of 5.8%.
考虑到卵巢癌的高发率和死亡率，尤其是在欧洲女性人群中，糖链抗原 125（CA-125）被选为该研究的蛋白质靶标，以开发基于分子印迹聚合物（MIP）的生物传感器。本工作介绍了在金电极表面制备用于 CA-125 生物标志物识别的分子印迹聚合物（MIP）。通过在金电极上使用循环伏安法（CV）聚合吡咯（Py）单体来制备 CA-125 印迹，以获得具有高选择性和出色分子识别能力的材料。CA-125 生物标志物的定量是通过比较两种方法来实现的：电化学（方波伏安法-SWV）和光学转换（表面等离子体共振-SPR）。SWV 已广泛用于生物分子分析，因为它是一种快速且灵敏的技术。反过来，SPR 是一种非破坏性的光学技术，可提供 CA-125 生物标志物与 MIP 相互作用的高质量分析数据。确定了几个分析参数，例如灵敏度、线性响应区间和检测限，以对用于 CA-125 生物传感器开发的电化学和光学转换的性能进行评估。基于电化学转换的生物传感器是呈现最佳分析参数的传感器，具有良好的选择性和检测限（LOD）为 0.01 U/mL，提供了 0.01 至 500 U/mL 的线性浓度范围。选择该电化学生物传感器进行研究，并成功地应用于人工血清样品中的 CA-125 分析，回收率在 91%至 105%之间，平均相对误差为 5.8%。
