Cardoso Ana Rita Aires, Barquinha Pedro Miguel Cândido, Sales Maria Goreti Ferreira
BioMark@UC/CEMMPRE (Centre for Mechanical Engineering, Materials and Processes)-ARISE (Advanced Production and Intelligent Systems), Faculty of Sciences and Technology, Department of Chemical Engineering, University of Coimbra, 3030-790 Coimbra, Portugal.
CENIMAT|i3N, Department of Materials Science, School of Science and Technology, NOVA University Lisbon and CEMOP/UNINOVA, Campus de Caparica, 2829-516 Caparica, Portugal.
Biosensors (Basel). 2025 Aug 15;15(8):537. doi: 10.3390/bios15080537.
This work describes a non-enzymatic electrochemical glucose biosensor combining for the first time molecularly imprinted polymers (MIPs) for glucose concentration and gold nanoparticles (AuNPs) on screen-printed carbon electrodes (SPEs), where both MIPs and AuNPs were assembled in situ. Electrochemical impedance spectroscopy (EIS) was used to evaluate the analytical performance of the sensor, which has a linear range between 1.0 µM and 1.0 mM when standard solutions are prepared in buffer. Direct measurement of glucose was performed by chronoamperometry, measuring the oxidation current generated during direct glucose oxidation. The selectivity was tested against ascorbic acid and the results confirmed a selective discrimination of the electrode for glucose. Overall, the work presented here represents a promising tool for tracking glucose levels in serum. The use of glucose MIP on the electrode surface allows the concentration of glucose, resulting in lower detection limits, and the use of AuNPs reduces the potential required for the oxidation of glucose, which increases selectivity. In addition, this possible combination of two analytical measurements following different theoretical concepts can contribute to the accuracy of the analytical measurements. This combination can also be extended to other biomolecules that can be electrochemically oxidised at lower potentials.
这项工作描述了一种非酶电化学葡萄糖生物传感器，该传感器首次将用于葡萄糖浓度检测的分子印迹聚合物（MIP）和丝网印刷碳电极（SPE）上的金纳米颗粒（AuNP）相结合，其中MIP和AuNP均原位组装。采用电化学阻抗谱（EIS）评估传感器的分析性能，当在缓冲液中制备标准溶液时，该传感器的线性范围为1.0 μM至1.0 mM。通过计时电流法直接测量葡萄糖，测量直接葡萄糖氧化过程中产生的氧化电流。针对抗坏血酸测试了选择性，结果证实该电极对葡萄糖具有选择性识别能力。总体而言，本文介绍的工作是一种用于跟踪血清中葡萄糖水平的有前景的工具。在电极表面使用葡萄糖MIP可实现葡萄糖的浓缩，从而降低检测限，而使用AuNP可降低葡萄糖氧化所需的电位，提高选择性。此外，遵循不同理论概念的两种分析测量方法的这种可能组合有助于提高分析测量的准确性。这种组合还可扩展到其他能在较低电位下进行电化学氧化的生物分子。
