Watanabe Y, Okura A, Naito K, Kobayashi M
Exploratory Research Laboratories, Banyu Pharmaceutical Co., Ltd, Tokyo.
Jpn J Cancer Res. 1988 Nov;79(11):1208-16. doi: 10.1111/j.1349-7006.1988.tb01546.x.
A reproducible tumor model for liver metastasis has been developed from murine L5178Y lymphoma line by sequential cycles of subcutaneous inoculation of liver tumor cells, that were originally generated in livers of female (BALB/c x DBA/2)F1 mice by injecting the parental cells into the tail vein. This variant (L5178Y-ML) metastasized predominantly to the liver after intravenous or subcutaneous injection. The livers of the animals killed 9 days after intravenous implantation of 5 x 10(5) tumor cells were about 3 times the weight of control livers. All tumor-bearing mice died 10 to 12 days after inoculation. Subcutaneous implantation of L5178Y-ML in the side flank of mice induced metastatic nodules spontaneously in the livers. The tumor cells proliferated more in livers than in the implanted sites, compared with the parental L5178Y cells. The effects of 5-fluorouracil, mitomycin C, cis-platinum and doxorubicin on the liver metastasis of L5178Y-ML were examined at subtoxic doses; 5-fluorouracil was the most effective in both inhibiting the tumor growth in livers and prolonging the survival period of mice. This model provides a useful tool for the experimental therapy of hepatic tumors in mice.
通过对最初由将亲代细胞经尾静脉注射到雌性（BALB/c×DBA/2）F1小鼠肝脏中产生的肝肿瘤细胞进行皮下接种的连续循环，已从鼠L5178Y淋巴瘤细胞系建立了一种可重复的肝转移肿瘤模型。这种变体（L5178Y-ML）在静脉内或皮下注射后主要转移至肝脏。在静脉内植入5×10⁵个肿瘤细胞9天后处死的动物肝脏重量约为对照肝脏的3倍。所有荷瘤小鼠在接种后10至12天死亡。在小鼠侧腹皮下植入L5178Y-ML可自发诱导肝脏出现转移结节。与亲代L5178Y细胞相比，肿瘤细胞在肝脏中的增殖比在植入部位更多。以亚毒性剂量检测了5-氟尿嘧啶、丝裂霉素C、顺铂和阿霉素对L5178Y-ML肝转移的影响；5-氟尿嘧啶在抑制肝脏肿瘤生长和延长小鼠生存期方面最为有效。该模型为小鼠肝脏肿瘤的实验治疗提供了一种有用的工具。
