Postgraduate Program in Biochemistry Sciences, Sector of Biological Sciences, Federal University of Paraná, Curitiba, PR, 81531-990, Brazil.
Division of Glycoscience, Royal Institute of Technology - KTH, Sweden; Department of Entomology and Nematology, University of Florida, Gulf Coast Research and Education Center (GCREC-UF), Wimauma, USA.
Carbohydr Polym. 2019 Nov 15;224:115140. doi: 10.1016/j.carbpol.2019.115140. Epub 2019 Aug 2.
A new source of pectin with a cytotoxic effect on glioblastoma cells is presented. A homogeneous GWP-FP-S fraction (M of 29,170 g mol) was obtained by fractionating the crude pectin extract (GW) from Campomanesia xanthocarpa pulp. According to the monosaccharide composition, the GWP-FP-S was composed of galacturonic acid (58.8%), arabinose (28.5%), galactose (11.3%) and rhamnose (1.1%), comprising 57.7% of homogalacturonans (HG) and 42.0% of type I rhamnogalacturonans (RG-I). These structures were characterized by chromatographic and spectroscopic methods; GW and GWP-FP-S fractions were evaluated by MTT and crystal violet assays for their cytotoxic effects. Both fractions induced cytotoxicity (15.55-37.65%) with concomitant increase in the cellular ROS levels in human glioblastoma cells at 25-400 μg mL, after 48 h of treatment, whereas no cytotoxicity was observed for normal NIH 3T3 cells. This is the first report of in vitro bioactivity and the first investigation of the antitumor potential of gabiroba pectins.
一种具有细胞毒性作用的新型果胶来源被提出。通过从 Campomanesia xanthocarpa 果肉中分离粗果胶提取物 (GW)，获得了均一的 GWP-FP-S 级分（M 为 29,170 g/mol）。根据单糖组成，GWP-FP-S 由半乳糖醛酸（58.8%）、阿拉伯糖（28.5%）、半乳糖（11.3%）和鼠李糖（1.1%）组成，包含 57.7%的同质半乳糖醛酸聚糖（HG）和 42.0%的 I 型鼠李半乳糖醛酸聚糖（RG-I）。这些结构通过色谱和光谱方法进行了表征；通过 MTT 和结晶紫测定法评估了 GW 和 GWP-FP-S 级分对人神经胶质瘤细胞的细胞毒性作用。两种级分在 25-400 μg/mL 浓度下，处理 48 小时后，均能诱导人神经胶质瘤细胞的细胞毒性（15.55-37.65%），同时细胞内 ROS 水平升高，而正常 NIH 3T3 细胞则没有观察到细胞毒性。这是首次报道了体外生物活性和巴西莓果胶的抗肿瘤潜力的研究。
