Wang Hui, Wang Jin, Yang Dan-Dan, Liu Zong-Li, Zeng Yong-Qing, Chen Wei
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271000, China.
Asian-Australas J Anim Sci. 2020 Mar;33(3):390-397. doi: 10.5713/ajas.18.0225. Epub 2019 Jul 1.
The objective of this study was to measure the special expression pattern of lipid metabolism genes and investigate the molecular mechanisms underlying intramuscular fat (IMF) deposition in Longissimus dorsi muscle of Laiwu pigs.
Thirty-six pigs (Laiwu n = 18; Duroc×Landrace×Yorkshire n = 18) were used for the measurement of the backfat thickness, marbling score, IMF content, and expression of lipid metabolism genes.
Significant correlations were found between IMF content and the mRNA expression of lipid metabolism genes. Of the 14 fat deposition genes measured, fatty acid synthase (FASN) showed the strongest correlation (r = 0.75, p = 0.001) with IMF content, and of the 6 fat removal genes, carnitine palmitoyl transferase 1B (CPT1B) exhibited the greatest negative correlation (r = -0.66, p = 0.003) with IMF content in Laiwu pig. Multiple regression analysis showed that CPT1B, FASN, solute carrier family 27 member 1 (SLC27A1), and fatty acid binding protein 3 (FABP3) contributed 38% of the prediction value for IMF content in Laiwu pigs. Of these four variables, CPT1B had the greatest contribution to IMF content (14%) followed by FASN (11%), SLC27A1 (9%), and FABP3 (4%).
Our results indicate that the combined effects of an upregulation in fat deposition genes and downregulation in fat removal genes promotes IMF deposition in Laiwu pigs.
本研究旨在测定脂质代谢基因的特殊表达模式，并探究莱芜猪背最长肌肌内脂肪（IMF）沉积的分子机制。
选用36头猪（莱芜猪18头；杜洛克×长白×大白猪18头）测量背膘厚度、大理石纹评分、IMF含量以及脂质代谢基因的表达。
发现IMF含量与脂质代谢基因的mRNA表达之间存在显著相关性。在所测定的14个脂肪沉积基因中，脂肪酸合酶（FASN）与IMF含量的相关性最强（r = 0.75，p = 0.001）；在所测定的6个脂肪清除基因中，肉碱棕榈酰转移酶1B（CPT1B）与莱芜猪IMF含量的负相关性最大（r = -0.66，p = 0.003）。多元回归分析表明，CPT1B、FASN、溶质载体家族27成员1（SLC27A1）和脂肪酸结合蛋白3（FABP3）对莱芜猪IMF含量预测值的贡献率为38%。在这四个变量中，CPT1B对IMF含量的贡献率最大（14%），其次是FASN（11%）、SLC27A1（9%）和FABP3（4%）。
我们的结果表明，脂肪沉积基因上调和脂肪清除基因下调的联合作用促进了莱芜猪的IMF沉积。
