State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang) , Nanchang University , Nanchang 330047 , China.
Shenzhen Longgang District Maternity & Child Healthcare Hospital , Shenzhen 518100 , China.
J Agric Food Chem. 2019 Sep 25;67(38):10614-10623. doi: 10.1021/acs.jafc.9b03430. Epub 2019 Sep 17.
Type 2 diabetes (T2D) is a pandemic disease chiefly characterized by hyperglycemia. In this study, the combination of serum lipidomic and metabolomic approach was employed to investigate the effect of arabinoxylan on type 2 diabetic rats and identify the critical biomarkers of T2D. Metabolomics analysis revealed that branched-chain amino acids, 12α-hydroxylated bile acids, ketone bodies, and several short- and long-chain acylcarnitines were significantly increased in T2D, whereas lysophosphatidylcholines (LPCs) were significantly decreased. Lipidomics analysis indicated T2D-related dyslipidemia was mainly associated with the increased levels of acetylcarnitine, free fatty acids (FFA), diacylglycerols, triacylglycerols, and cholesteryl esters and the decreased levels of some unsaturated phosphatidylcholines (less than 22 carbons). These variations indicated the disturbed amino acid and lipid metabolism in T2D, and the accumulation of incompletely oxidized lipid species might eventually contribute to impaired insulin action and glucose homeostasis. Arabinoxylan treatment decreased the concentrations of 12α-hydroxylated bile acids, carnitines, and FFAs and increased the levels of LPCs. The improved bile acid and lipid metabolism by arabinoxylan might be involved in the alleviation of hypercholesterolemia and hyperlipidemia in T2D.
2 型糖尿病（T2D）是一种主要以高血糖为特征的流行疾病。在这项研究中，采用血清脂质组学和代谢组学方法相结合的方法，研究阿拉伯木聚糖对 2 型糖尿病大鼠的影响，并鉴定 T2D 的关键生物标志物。代谢组学分析显示，支链氨基酸、12α-羟化胆酸、酮体和几种短链和长链酰基肉碱在 T2D 中显著增加，而溶血磷脂酰胆碱（LPC）显著减少。脂质组学分析表明，T2D 相关的血脂异常主要与乙酰肉碱、游离脂肪酸（FFA）、二酰甘油、三酰甘油和胆固醇酯水平升高以及某些不饱和磷脂酰胆碱（少于 22 个碳）水平降低有关。这些变化表明 T2D 中氨基酸和脂质代谢紊乱，不完全氧化的脂质种类的积累可能最终导致胰岛素作用和葡萄糖稳态受损。阿拉伯木聚糖治疗降低了 12α-羟化胆酸、肉碱和 FFA 的浓度，并增加了 LPC 的水平。阿拉伯木聚糖改善的胆汁酸和脂质代谢可能参与缓解 T2D 的高胆固醇血症和高脂血症。
