Mahboob Shahid, Al-Ghanim K A, Al-Misned F, Al-Balawi H F Alkahem, Ashraf Asma, Al-Mulhim Norah M A
Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh, Saudi Arabia.
Department of Zoology, Government College University, Faisalabad, Pakistan.
Saudi J Biol Sci. 2019 Feb;26(2):363-367. doi: 10.1016/j.sjbs.2018.11.015. Epub 2018 Nov 22.
Genetic variation in fish stocks decreasing due to water pollution in the freshwater rivers, streams and canals. The objective of this study was to determine the genetic diversity and polymorphism in collected from the Wadi Hanefah Riyadh, Saudi Arabia by using RAPD-PCR. Total thirty fish specimens were harvested from each of four pre-determined locations of the reservoir which were designated as H1, H2, H3, and H4. Five random decamer primers were used to assess the diversity in the stock of In this fish stock 48 bands were polymorphic and 12 were monomorphic. The maximum polymorphism (100%) was recorded in the fish samples procured from H4, followed by 88.75, 87.33 and 76.12% of the tilapia collected from H3, H2, and H4, respectively. Nei's genetic distance value was ranged as 0.0005 to 0.1006. Maximum and minimum genetic distance was recorded as 0.1006 and 0.005 in tilapia harvested from H1 and H2 locations. Average heterozygosity was ranged from 0.3009 to 0.3744. This information about the genetic polymorphism of may be used by the concerned authorities to evolve strategies to conserve the diversity of tilapia in the country.
由于淡水河流、溪流和运河中的水污染，鱼类种群的遗传变异正在减少。本研究的目的是通过随机扩增多态性DNA聚合酶链式反应（RAPD-PCR）来确定从沙特阿拉伯利雅得瓦迪哈尼法采集的罗非鱼的遗传多样性和多态性。从水库预先确定的四个地点（分别指定为H1、H2、H3和H4）中的每一个地点收获了总共30个鱼类标本。使用五个随机十聚体引物来评估罗非鱼种群的多样性。在这个罗非鱼种群中，48条带是多态性的，12条是单态性的。在从H4采集的鱼样本中记录到最大多态性（100%），其次是分别从H3、H2和H1采集的罗非鱼的88.75%、87.33%和76.12%。内氏遗传距离值范围为0.0005至0.1006。在从H1和H2地点收获的罗非鱼中，最大和最小遗传距离分别记录为0.1006和0.005。平均杂合度范围为0.3009至0.3744。有关当局可利用这些关于罗非鱼遗传多态性的信息来制定保护该国罗非鱼多样性的策略。
