Department of Medical Biochemistry, Wroclaw Medical University, Chalubinskiego 10, 50-368 Wroclaw, Poland.
Department of Fruit, Vegetable and Plant Nutraceutical Technology, Wroclaw University of Environmental and Life Sciences, J. Chelmonskiego 37, 51-630 Wroclaw, Poland.
Molecules. 2022 Jun 29;27(13):4193. doi: 10.3390/molecules27134193.
Despite the fact that phytochemicals of species have long been discussed as possible auxiliary agents in contemporary treatment, the insights on their properties remain relatively scarce. This study focuses on L. (Cornelian cherry), the extracts of which are reported to exert a pleiotropic effect shown in both in vivo and in vitro studies. This study aimed to explore the cytotoxic effect of extracts from fruits of red ( L. 'Podolski') and yellow ( L. 'Yantarnyi' and 'Flava') Cornelian cherries on two melanoma cell lines (A375 and MeWo). The extracts were characterized in the context of the concentration of bioactive compounds of antioxidative properties. Cytotoxicity was investigated with the use of the following two assays: SRB and MTT. An additional, alternative protocol for the SRB assay was used in this study so as to account for possible bias. Cytotoxicity was assessed as a difference in the whole time series of cell viability, instead of analyzing differences in raw values (often found in the literature). Both extracts from L. induced cytotoxicity in both A375 and MeWo cell lines, although the response of these cells was different. Moreover, based on this study, there is no evidence for claiming a different magnitude of cytotoxicity between these two extracts.
尽管 物种的植物化学物质长期以来一直被讨论为当代治疗的可能辅助剂，但对其特性的了解仍然相对较少。本研究集中在 L.（山茱萸）上，据报道，其提取物在体内和体外研究中均表现出多效作用。本研究旨在探索红色（ L. '波德尔斯基'）和黄色（ L. '扬塔尔尼'和'弗拉瓦'）山茱萸果实提取物对两种黑色素瘤细胞系（A375 和 MeWo）的细胞毒性作用。提取物的特征在于抗氧化特性的生物活性化合物的浓度。使用以下两种测定法研究细胞毒性：SRB 和 MTT。本研究中使用了另一种替代的 SRB 测定法方案，以消除可能的偏差。细胞毒性被评估为细胞活力的整个时间序列的差异，而不是分析原始值的差异（在文献中经常发现）。来自 L. 的两种提取物都诱导了 A375 和 MeWo 细胞系的细胞毒性，尽管这些细胞的反应不同。此外，基于这项研究，没有证据表明这两种提取物的细胞毒性有不同的程度。
