Charoensiri S, Katoh A K
Immunol Commun. 1979;8(4):407-18. doi: 10.3109/08820137909050054.
The role of macrophages in the transformation of human lymphocytes by the mitogen galactose oxidase was studied. Monocyte depleted, purified T or B cells did not undergo blastogenesis after treatment with galactose oxidase. When galactose oxidase-treated purified T cells were cultured with macrophages, a slight proliferative response was obtained. B cells treated similarly showed no response. When macrophages were treated with galactose oxidase, and then T & B, T, or B cells were added, proliferative responses were observed in all 3 categories. Finally, supernatants of media in which galactose oxidase-treated macrophages were cultured also demonstrated the property of stimulating lymphocyte transformation. These results are consistent with current concepts of mitogen "presentation" and the elaboration of soluble factors by macrophages in mediating the activation of lymphocytes in response to stimulating agents.
研究了巨噬细胞在有丝分裂原半乳糖氧化酶诱导人淋巴细胞转化过程中的作用。去除单核细胞的纯化T细胞或B细胞在用半乳糖氧化酶处理后未发生母细胞化。当用半乳糖氧化酶处理过的纯化T细胞与巨噬细胞共同培养时，可获得轻微的增殖反应。经类似处理的B细胞无反应。当用半乳糖氧化酶处理巨噬细胞，然后加入T细胞和B细胞、T细胞或B细胞时，在所有这三类细胞中均观察到增殖反应。最后，培养过用半乳糖氧化酶处理过的巨噬细胞的培养基上清液也显示出刺激淋巴细胞转化的特性。这些结果与有丝分裂原“呈递”以及巨噬细胞分泌可溶性因子介导淋巴细胞对刺激剂作出反应的激活作用的当前概念相一致。
