皮质类固醇对淋巴细胞对半乳糖氧化酶修饰淋巴细胞刺激反应的影响。
Effect of corticosteroids on the response of lymphocytes to stimulation by galactose oxidase-modified lymphocytes.
作者信息
Yu D T, Ramer S J, Kacena A
出版信息
Immunology. 1977 Aug;33(2):247-52.
Human peripheral blood mononuclear cell preparations, after treatment by neuraminidase plus galactose oxidase, stimulated untreated lymphocytes. The increases in tritiated thymidine incorporation in the responder lymphocytes were observed after 48 h of mixed cell cultures. Monocytedepleted lymphocyte preparations were equally effective stimulator cells. Both purified T and B fractions were effective stimulator cells. On the other hand, only the T but not the B fractions could respond to the stimulation. The response of the cells to this type of stimulation was suppressed by 10(4)-10(7) M of the corticosteroid preparation methylprednisolone. When the cells treated with neuraminidase plus galactose oxidase were cultured alone for 48 h, they lost their stimulating capacity. However this loss could not be prevented by the presence in the culture of methylprednisolone. Hence the drug has selective suppressive activity on one type of lymphocyte activity but not the other.
人外周血单个核细胞制剂经神经氨酸酶加半乳糖氧化酶处理后，可刺激未处理的淋巴细胞。混合细胞培养48小时后，观察到应答淋巴细胞中氚标记胸腺嘧啶核苷掺入量增加。去除单核细胞的淋巴细胞制剂同样是有效的刺激细胞。纯化的T细胞和B细胞组分都是有效的刺激细胞。另一方面，只有T细胞组分而非B细胞组分能对刺激作出反应。细胞对这种类型刺激的反应受到10⁴ - 10⁷ M皮质类固醇制剂甲泼尼龙的抑制。当用神经氨酸酶加半乳糖氧化酶处理的细胞单独培养48小时后，它们失去了刺激能力。然而，培养物中存在甲泼尼龙并不能阻止这种能力的丧失。因此，该药物对一种淋巴细胞活性具有选择性抑制活性，而对另一种则没有。
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