Department of Gastroenterology, First Medical Center, Chinese People's Liberation Army General Hospital, Beijing, China.
Department of Gastroenterology, Second Medical Center, Chinese People's Liberation Army General Hospital, Beijing, China.
Sci Rep. 2019 Sep 6;9(1):12874. doi: 10.1038/s41598-019-49415-3.
This study aimed to investigate the intestinal microbiota in duodenal ulcer (DU) patients, effects of proton pump inhibitors,clarithromycin and amoxicillin, PCA) for Helicobacter pylori (H. pylori) and Bacillus subtilis and Enterococcus faecium (BSEF) on intestinal microbiota. DU patients were randomly assigned to receive either PCA (group TT) or PCA plus BSEF(group TP). The fecal microbiome was conducted using high throughput 16S rDNA gene and internal transcribed spacer sequencings. The diversity and abundance of intestinal bacteria in the DU were significantly lower than health check control (HC) group. In the TT group, the abundance and diversity of both intestinal bacteria and fungi decreased after PCA treatment, compared with those before treatment, whereas in the TP group no obvious changes were observed. In the TT group at all the time points, both the intestinal bacteria and fungi were different from those in the HC group. However, in the TP group, at 10w the bacterial flora abundance was close to that in the HC group. The results indicate that anti- H. pylori treatment induced significant decrease in the diversity of intestinal microbiota, while the combined therapy supplemented with BSEF could protect and restore the intestinal microbiota.
本研究旨在探讨十二指肠溃疡（DU）患者的肠道微生物群，质子泵抑制剂、克拉霉素和阿莫西林（PCA）对幽门螺杆菌（H. pylori）和枯草芽孢杆菌及屎肠球菌（BSEF）的影响，以及 PCA 加 BSEF（TP 组）对肠道微生物群的影响。将 DU 患者随机分为接受 PCA（TT 组）或 PCA 加 BSEF（TP 组）治疗。采用高通量 16S rDNA 基因和内部转录间隔区测序技术对粪便微生物群进行检测。与健康检查对照组（HC 组）相比，DU 患者肠道细菌的多样性和丰度明显较低。在 TT 组中，与治疗前相比，PCA 治疗后肠道细菌和真菌的丰度和多样性均降低，而在 TP 组中未观察到明显变化。在 TT 组的所有时间点，肠道细菌和真菌均与 HC 组不同。然而，在 TP 组中，10 周时细菌菌群丰度接近 HC 组。结果表明，抗 H. pylori 治疗可显著降低肠道微生物群的多样性，而补充 BSEF 的联合治疗可保护和恢复肠道微生物群。
