首次从犬脓皮病分离的中间葡萄球菌中检测到来自肠球菌属的多耐药 pRE25 样元件。

First detection of multiresistance pRE25-like elements from Enterococcus spp. in Staphylococcus pseudintermedius isolated from canine pyoderma.

机构信息

Laboratory of Veterinary Dermatology and The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 08826, South Korea.

出版信息

J Glob Antimicrob Resist. 2020 Mar;20:304-308. doi: 10.1016/j.jgar.2019.08.022. Epub 2019 Sep 4.

DOI:10.1016/j.jgar.2019.08.022

PMID:31493528

Abstract

OBJECTIVES

Enterococcus pRE25 is a conjugative and mobilising multiresistance plasmid from Enterococcus faecalis RE25. pRE25-like enterococcal plasmid pWZ909 mediates the delivery of vancomycin resistance to methicillin-resistant Staphylococcus aureus via a Tn1546-like transposon. However, whether pRE25-like elements contribute to multidrug resistance in Staphylococcus spp. has not yet been investigated. Here we describe the first detection of multiresistance pRE25-like elements in the chromosomal DNA of multidrug-resistant Staphylococcus pseudintermedius (MDRSP).

METHODS

A total of 46 MDRSP clinical strains were isolated from canine pyoderma in Korea. Their genetic characteristics were analysed by multilocus sequence typing (MLST) and PCR targeting pRE25-like elements. Whole-genome sequencing (WGS) was performed on four isolates.

RESULTS

WGS detected that the chromosomal 22-kb pRE25-like elements contained five antimicrobial resistance genes [cat, erm(B), aphA-3, aadK and sat4], IS1252, IS256, and a toxin-antitoxin system within copies of IS1216. BLASTn alignment analysis revealed that 84% of the chromosomal 22-kb pRE25-like elements sequence is homologous (99.8% identity) to the enterococcal pRE25 plasmid sequence. PCR assay showed that 52.2% of MDRSP isolates were positive for pRE25-like elements and were presumed to contain pRE25-like elements (pRE25 group). The sequence types (STs) of the pRE25 group were diverse, with 18 STs identified, among which 12 STs were first reported in Korea.

CONCLUSION

Enterococcal pRE25-like elements are suspected to be widespread in MDRSP isolated from companion dogs in Korea. Considering that companion dogs live in a closely shared environment with humans, continuous surveillance of pRE25-like elements is needed for other staphylococci commonly isolated from humans.

摘要

目的

肠球菌 pRE25 是一种来自粪肠球菌 RE25 的可接合和可移动的多药耐药质粒。pRE25 样肠球菌质粒 pWZ909 通过 Tn1546 样转座子介导万古霉素耐药性向耐甲氧西林金黄色葡萄球菌的传递。然而，pRE25 样元件是否有助于葡萄球菌属的多药耐药性尚未得到研究。在这里，我们描述了首次在多药耐药性中间型葡萄球菌（MDRSP）的染色体 DNA 中检测到多药耐药性 pRE25 样元件。

方法

从韩国犬脓皮病中分离出 46 株 MDRSP 临床株。通过多位点序列分型（MLST）和针对 pRE25 样元件的 PCR 分析其遗传特征。对 4 个分离物进行全基因组测序（WGS）。

结果

WGS 检测到染色体 22kb pRE25 样元件包含 5 个抗菌药物耐药基因 [cat、erm(B)、aphA-3、aadK 和 sat4]、IS1252、IS256 和 IS1216 拷贝内的毒素-抗毒素系统。BLASTn 比对分析显示，染色体 22kb pRE25 样元件序列的 84%（99.8% 同一性）与肠球菌 pRE25 质粒序列同源。PCR 检测显示，52.2%的 MDRSP 分离株 pRE25 样元件阳性，推测含有 pRE25 样元件（pRE25 组）。pRE25 组的序列型（ST）多种多样，共鉴定出 18 个 ST，其中 12 个 ST 是韩国首次报道的。