Institute of Chemical Biology and Nanomedicine (ICBN), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, People's Republic of China.
College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, People's Republic of China.
Mikrochim Acta. 2019 Sep 7;186(10):675. doi: 10.1007/s00604-019-3762-5.
Nitrogen-doped carbon dots (N-CDs) with fluorescence excitation/emission maxima at 365/450 nm were prepared by a one-step hydrothermal approach. The dots possess remarkable photostability, fluorescence blinking and good biocompatibility, and this favors utilization in stochastic optical reconstruction microscopy (STORM). A spatial resolution down to ~60 nm was achieved when imaging HeLa cells using 647-nm laser excitation. This opens new possibilities for various super-resolution techniques based on stochastic optical switching. The remarkable optical properties of the N-CDs also enable them to be applied as invisible security ink for use in patterning, information storage and anti-counterfeiting. Further, it is found that the fluorescence of the N-CDs can be quenched by curcumin with high efficiency due to a combination of inner filter effect and static quenching. Based on this, a fluorometric assay with a detection limit of 21 ng mL was developed for the determination of curcumin. Graphical abstract Schematic representation of the applications of N-doped carbon dots (N-CDs). Curcumin quenches the fluorescence of N-CDs with high efficiency. The remarkable optical properties of the N-CDs enable them to be applied in fluorescent ink, cell imaging and stochastic optical reconstruction microscopy (STORM).
氮掺杂碳点（N-CDs）具有荧光激发/发射最大值为 365/450nm，采用一步水热法制备。这些点具有显著的光稳定性、荧光闪烁和良好的生物相容性，这有利于在随机光学重建显微镜（STORM）中使用。当使用 647nm 激光激发对 HeLa 细胞进行成像时，实现了低至约 60nm 的空间分辨率。这为基于随机光学开关的各种超分辨率技术开辟了新的可能性。N-CDs 的显著光学性质还使它们能够用作隐形安全油墨，用于图案化、信息存储和防伪。此外，还发现由于内滤效应和静态猝灭的组合，姜黄素可以高效地猝灭 N-CDs 的荧光。基于此，开发了一种荧光测定法，检测限为 21ng mL，用于测定姜黄素。图表摘要 N 掺杂碳点（N-CDs）应用示意图。姜黄素高效猝灭 N-CDs 的荧光。N-CDs 的显著光学性质使其能够应用于荧光油墨、细胞成像和随机光学重建显微镜（STORM）。
