Zan Minghui, Li Cong, Liao Fei, Rao Lang, Meng Qian-Fang, Xie Wei, Chen Bei, Qie Xingwang, Li Li, Wang Liang, Dong Wen-Fei, Liu Wei
Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University Wuhan Hubei 430072 China
Shenzhen Research Institute of Wuhan University Shenzhen Guangdong 518057 China.
RSC Adv. 2020 Mar 9;10(17):10067-10075. doi: 10.1039/c9ra11009g. eCollection 2020 Mar 6.
In recent years, carbon dot (CD)-based fluorescent sensors for selective ions or small biomolecules have drawn great attention. In this work, highly fluorescent CDs (QY = 21%) were prepared from 2,3-diamino pyridine as the precursor through a facile solvothermal process. The CDs showed high stability and a green emission in aqueous, and the optimal emission wavelength of CDs is 508 nm under the excitation wavelength of 438 nm. Interestingly, a CDs-based nanoprobe was developed for a selective and sensitive fluorescence quenching response to NO in water, and the quenching mechanism was investigated in the work. Besides, the recovery rates of NO in the range of 98-103.5% were found to be acceptable, indicating that the proposed CDs could be act as potential candidates for determination of nitrite ions in real samples. Meanwhile, the nanoprobe was also successfully employed in a visualization biosensing platform for determination of NO in living cells due to its eminent biocompatibility.
近年来，用于选择性检测离子或小分子的基于碳点（CD）的荧光传感器备受关注。在这项工作中，以2,3 - 二氨基吡啶为前驱体，通过简便的溶剂热法制备了高荧光碳点（量子产率QY = 21%）。这些碳点在水溶液中表现出高稳定性和绿色发射，在438 nm激发波长下，碳点的最佳发射波长为508 nm。有趣的是，开发了一种基于碳点的纳米探针，用于对水中的NO进行选择性和灵敏的荧光猝灭响应，并在该工作中研究了猝灭机制。此外，发现NO在98 - 103.5%范围内的回收率是可接受的，这表明所提出的碳点可作为测定实际样品中亚硝酸根离子的潜在候选物。同时，由于其卓越的生物相容性，该纳米探针还成功应用于可视化生物传感平台，用于测定活细胞中的NO。
