College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, China; Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Microb Pathog. 2019 Dec;137:103736. doi: 10.1016/j.micpath.2019.103736. Epub 2019 Sep 7.
Respiratory infections such as SARS-CoV in humans are often accompanied by mild and self-limiting hepatitis. As a respiratory disease, influenza A virus (IAV) infection can lead to hepatitis, but the mechanism remains unclear. This study aimed to investigate the occurrence of hepatitis by establishing a model for infected mice for three different subtypes of respiratory IAVs (H1N1, H5N1, and H7N2). Histological analysis was performed, and results showed increase serum aminotransferase (ALT and AST) levels and evident liver injury on days 3 and 7, especially on day 5 post infection. Immunohistochemistry (IHC) results indicated a wide distribution of IAV's positive signals in the liver of infected mice. Real-time PCR results further revealed a similar viral titer to IHC that presented a remarkedly positive correlation with histology injury. All these data showed that the mouse model suitably contributed valuable information about the mechanism underlying the occurrence of hepatitis induced by respiratory influenza virus.
人类呼吸道感染，如 SARS-CoV，常伴有轻微且自限性的肝炎。作为一种呼吸道疾病，甲型流感病毒（IAV）感染可导致肝炎，但机制尚不清楚。本研究旨在通过建立感染三种不同亚型呼吸道 IAV（H1N1、H5N1 和 H7N2）的小鼠模型，探讨肝炎的发生情况。进行了组织学分析，结果显示，在感染后第 3 天和第 7 天，血清转氨酶（ALT 和 AST）水平升高，肝脏损伤明显，特别是在感染后第 5 天。免疫组织化学（IHC）结果表明，IAV 的阳性信号在感染小鼠的肝脏中广泛分布。实时 PCR 结果进一步表明，病毒滴度与 IHC 相似，与组织学损伤呈显著正相关。所有这些数据表明，该小鼠模型为呼吸道流感病毒引起肝炎的发生机制提供了有价值的信息。
