Mohamadimaram Mohamadreza, Allahbakhshian Farsani Mehdi, Mirzaeian Amin, Shahsavan Shaghayegh, Hajifathali Abbass, Parkhihdeh Sayeh, Mohammadi Mohammad Hossein
Laboratory Hematology and blood Banking Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
HSCT Research Center, Laboratory Hematology and blood Banking Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2019 Spring;18(2):1060-1066. doi: 10.22037/ijpr.2019.1100682.
Autophagy, known as cell death type II, is a housekeeping pathway that currently has been worked on in matters of tumorigenesis and leukomogenesis. Therefore, expression levels of ATG7 and LC3 as two key genes in AML patients are targeted in this study.
This study was performed on 55 de novo AML patients against 17 healthy volunteers, acquired samples from bone marrow (BM) and peripheral blood (PB) sources in different ages and gender. The evaluation was executed by mRNA extraction, cDNA synthesis, real-time PCR and data was analyzed by SPSS.
Analyzed data indicate a significant decrease between expression of ATG7 and LC3 in AML patients against control (Pv < 0.05). Decrease in both genes expression was detected in most of the patients, 81.81% and 75.55%, respectively. Also LC3 overexpression was detected in 11.33% of AML patients. Moreover, a positive significant correlation between ATG7 and LC3 genes was detected (r = 0.481; Pv = 0.001).
This study showed that significant reduction of autophagy genes in de novo AML patients is important to overcome this system and initiate leukomogenesis. It seems a new insight is required for new achievements in diagnosis, prognosis, treatment and monitoring AML patients.
自噬，即II型细胞死亡，是一种细胞维持机制，目前在肿瘤发生和白血病发生方面已有相关研究。因此，本研究针对急性髓系白血病（AML）患者中两个关键基因ATG7和LC3的表达水平展开研究。
本研究选取了55例初发AML患者，并与17名健康志愿者进行对照，采集了不同年龄和性别的骨髓（BM）和外周血（PB）样本。通过mRNA提取、cDNA合成及实时定量PCR进行评估，并使用SPSS软件分析数据。
分析数据表明，AML患者中ATG7和LC3的表达与对照组相比显著降低（Pv < 0.05）。大多数患者（分别为81.81%和75.55%）的这两个基因表达均下降。此外，在11.33%的AML患者中检测到LC3过表达。而且，检测到ATG7和LC3基因之间存在显著正相关（r = 0.481；Pv = 0.001）。
本研究表明，初发AML患者自噬基因的显著降低对于突破该系统并引发白血病发生具有重要意义。在AML患者的诊断、预后、治疗及监测方面取得新进展似乎需要新的见解。
