Leibniz-Institut für Polymerforschung Dresden e. V., 01069 Dresden, Germany.
Institute of Anatomy and Cell Biology, Paracelsus Medical Private University, Salzburg and Nuremberg, 90419 Nuremberg, Germany.
Int J Mol Sci. 2019 Sep 19;20(18):4655. doi: 10.3390/ijms20184655.
A rupture of the anterior cruciate ligament (ACL) is the most common knee ligament injury. Current applied reconstruction methods have limitations in terms of graft availability and mechanical properties. A new approach could be the use of a tissue engineering construct that temporarily reflects the mechanical properties of native ligament tissues and acts as a carrier structure for cell seeding. In this study, embroidered scaffolds composed of polylactic acid (PLA) and poly(lactic-co-ε-caprolactone) (P(LA-CL)) threads were tested mechanically for their viscoelastic behavior under in vitro degradation. The relaxation behavior of both scaffold types (: mono-component scaffold made of PLA threads, : bi-component scaffold made of PLA and P(LA-CL) threads) was comparable to native lapine ACL. Most of the lapine ACL cells survived 32 days of cell culture and grew along the fibers. Cell vitality was comparable for and scaffolds. Lapine ACL cells were able to adhere to the polymer surfaces and spread along the threads throughout the scaffold. The mechanical behavior of degrading matrices with and without cells showed no significant differences. These results demonstrate the potential of embroidered scaffolds as an ACL tissue engineering approach.
前交叉韧带（ACL）撕裂是最常见的膝关节韧带损伤。目前应用的重建方法在移植物可用性和机械性能方面存在局限性。一种新的方法可能是使用组织工程构建体，该构建体暂时反映天然韧带组织的机械性能，并作为细胞接种的载体结构。在这项研究中，我们对由聚乳酸（PLA）和聚（丙交酯-共-ε-己内酯）（P（LA-CL））线组成的绣花支架进行了机械测试，以测试其在体外降解下的粘弹性行为。两种支架类型（：由 PLA 线制成的单组分支架，：由 PLA 和 P（LA-CL）线制成的双组分支架）的松弛行为与天然兔 ACL 相似。大多数兔 ACL 细胞在细胞培养 32 天后存活下来，并沿着纤维生长。和支架的细胞活力相当。兔 ACL 细胞能够附着在聚合物表面上，并沿着整个支架的纤维展开。有细胞和无细胞的降解基质的机械性能没有显著差异。这些结果表明绣花支架作为 ACL 组织工程方法的潜力。
