Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China.
Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin, 150030, China.
J Dairy Sci. 2019 Dec;102(12):10838-10844. doi: 10.3168/jds.2019-16593. Epub 2019 Sep 20.
Lactobacillus plantarum J26, a significant probiotic isolated from Chinese traditional fermented dairy products, exerts a positive immunomodulatory effect by regulating the expression of immune-related genes. We investigated expression of the cytokines IL-1α, IL-1β, IL-6, and tumor necrosis factor-α in the intestinal tract of mice stimulated by L. plantarum J26. In vivo, these cytokines were upregulated, peaked on d 5, and then decreased to the control level, indicating that L. plantarum J26 could induce expression of the genes encoding these proinflammatory cytokines. Teichoic acids produced by L. plantarum are recognized as key immunomodulatory molecules involved in the regulation of the host immune response. To better understand the genetic basis of this immunomodulatory mechanism, we sequenced and analyzed the whole genome of L. plantarum J26. The genome of L. plantarum J26 contains a circular chromosome and 4 circular plasmids. Lactobacillus plantarum J26 was predicted to synthesize ribitol-type backbones of wall teichoic acid. Furthermore, orthologous average nucleotide identity (OrthoANI) values showed that the genome was highly similar (>98.00%) to other L. plantarum strains, especially to L. plantarum ST-III and JDM1. The genomic data of L. plantarum J26 provide a genetic basis to further elucidate its mechanism of immunoregulation and will facilitate its application in the functional dairy food industry.
植物乳杆菌 J26 是从中国传统发酵乳制品中分离出来的一种重要益生菌，通过调节免疫相关基因的表达发挥积极的免疫调节作用。我们研究了植物乳杆菌 J26 刺激小鼠肠道中细胞因子 IL-1α、IL-1β、IL-6 和肿瘤坏死因子-α的表达。在体内，这些细胞因子上调，在第 5 天达到峰值，然后下降到对照水平，表明植物乳杆菌 J26 可以诱导这些促炎细胞因子基因的表达。植物乳杆菌产生的磷壁酸被认为是参与宿主免疫反应调节的关键免疫调节分子。为了更好地了解这种免疫调节机制的遗传基础，我们对植物乳杆菌 J26 的全基因组进行了测序和分析。植物乳杆菌 J26 的基因组包含一个圆形染色体和 4 个圆形质粒。植物乳杆菌 J26 被预测合成壁磷壁酸的核糖醇型骨架。此外，直系同源平均核苷酸同一性（OrthoANI）值表明，基因组与其他植物乳杆菌菌株高度相似（>98.00%），尤其是与植物乳杆菌 ST-III 和 JDM1 相似。植物乳杆菌 J26 的基因组数据为进一步阐明其免疫调节机制提供了遗传基础，并将有助于其在功能性乳制品行业的应用。
