Toragall Makhadumsab M, Satapathy Sanat K, Kadadevaru Girish G, Hiremath Murigendra B
Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.
Hubli Assisted Conception Centre, Hubli, Karnataka, India.
J Hum Reprod Sci. 2019 Jul-Sep;12(3):199-203. doi: 10.4103/jhrs.JHRS_155_18.
Male infertility is a medical problem, attributed to 50% of infertility. Seminal plasma can be an anticipating factor as it comprises secretions of accessory sex gland, thus offering novel and precise ways to understand potential roles of these biochemical markers in male infertility.
The objective of this study was to assess the correlation between biochemical markers and sperm parameters in envisaging male infertility.
We enlisted 105 men with fertility issue as patients and 25 fertile men as controls to evaluate the sperm parameters and biochemical markers, namely fructose and citric acid in ascertaining male infertility.
The semen samples from patients were collected properly and analyzed according to the World Health Organization-2010 manual. Later samples were centrifuged, seminal plasma was collected, and biochemical markers assessment was carried out by standard protocols.
Descriptive statistics, independent -test, one-way ANOVA, and Pearson correlation were used for statistical analysis of different variables using SPSS 20.0. The mean sperm count and motility by all infertile conditions displayed a significant difference when compared with the controls ( < 0.05).
The mean fructose levels of oligozoospermia showed a nonsignificance difference when compared with controls ( < 0.05). Asthenozoospermia, asthenoteratozoospermia, and azoospermia had a significance difference ( < 0.05) for citric acid levels. Pearson correlation coefficient showed significant negative correlation of sperm count ( = -0.564) and sperm motility ( = -0.574) with fructose levels. Whereas seminal citric acid concentration had a positive correlation with sperm count ( = 0.458) and sperm motility ( = 0.446).
Therefore, evaluation of certain biochemical markers of seminal fluid may benefit in understanding the functionality of accessory glands which subsidizes significantly to the seminal volume.
男性不育是一个医学问题，占不孕症的50%。精浆可作为一个预测因素，因为它包含附属性腺的分泌物，从而为了解这些生化标志物在男性不育中的潜在作用提供了新颖而精确的方法。
本研究的目的是评估生化标志物与精子参数在预测男性不育方面的相关性。
我们招募了105名有生育问题的男性作为患者，以及25名生育能力正常的男性作为对照，以评估精子参数和生化标志物，即果糖和柠檬酸，以确定男性不育情况。
妥善收集患者的精液样本，并根据世界卫生组织2010年手册进行分析。随后对样本进行离心，收集精浆，并通过标准方案进行生化标志物评估。
使用SPSS 20.0对不同变量进行描述性统计、独立样本t检验、单因素方差分析和Pearson相关性分析。与对照组相比，所有不育情况的平均精子计数和活力均显示出显著差异（P<0.05）。
少精子症患者的平均果糖水平与对照组相比无显著差异（P>0.05）。弱精子症、弱畸精子症和无精子症患者的柠檬酸水平有显著差异（P<0.05）。Pearson相关系数显示精子计数（r=-0.564）和精子活力（r=-0.574）与果糖水平呈显著负相关。而精浆柠檬酸浓度与精子计数（r=0.458）和精子活力（r=0.446）呈正相关。
因此，评估精液中的某些生化标志物可能有助于了解附属腺的功能，附属腺对精液量有显著贡献。
