Benyajati C, Place A R, Powers D A, Sofer W
Proc Natl Acad Sci U S A. 1981 May;78(5):2717-21. doi: 10.1073/pnas.78.5.2717.
The gene that codes for Drosophila alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase EC 1.1.1.1) was identified in a bacteriophage lambda library of genomic Drosophila DNA by using ADH cDNA cloned DNA as a probe. The DNA sequence of the protein encoding region was shown to be in agreement with the amino acid sequence of the ADH. Two intervening DNA sequences (introns) were identified within the protein encoding region: one was 65 nucleotides and located between the codons for amino acid residues 32 and 33, and one was 70 nucleotides and located between the codons for amino acid residues 167 and 168. Both contained the 5' G-T and 3' A-G dinucleotides characteristic of intron boundaries of eukaryotic genes. On the basis of secondary structure predictions, the first 140 amino acid residues of Drosophila ADH are in an alternating beta-sheet/alpha-helix arrangement which is characteristic of the coenzyme binding domain of dehydrogenases. The smaller of the two introns interrupts the domain predicted to bind the adenine portion of the coenzyme.
利用克隆的果蝇乙醇脱氢酶（ADH；乙醇：NAD+氧化还原酶，EC 1.1.1.1）cDNA作为探针，在果蝇基因组DNA的λ噬菌体文库中鉴定出了编码该酶的基因。编码蛋白区域的DNA序列与ADH的氨基酸序列一致。在编码蛋白区域内鉴定出两个间隔DNA序列（内含子）：一个有65个核苷酸，位于氨基酸残基32和33的密码子之间；另一个有70个核苷酸，位于氨基酸残基167和168的密码子之间。两者都含有真核基因内含子边界特有的5' G-T和3' A-G二核苷酸。根据二级结构预测，果蝇ADH的前140个氨基酸残基呈交替的β-折叠/α-螺旋排列，这是脱氢酶辅酶结合域的特征。两个内含子中较小的一个打断了预测与辅酶腺嘌呤部分结合的结构域。
