Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Carbohydr Polym. 2019 Dec 15;226:115299. doi: 10.1016/j.carbpol.2019.115299. Epub 2019 Sep 7.
Chitosan-Bioactive glass (CS-BG) nanocomposite coatings were developed on the Ti-6Al-4 V alloy to investigate the effect of the BG content on the adhesion strength, bioactivity, bio-corrosion, wettability and roughness. For this purpose, BG nanoparticles were synthesized using a sol-gel process. Three nanocomposite coatings with different concentrations of BG (0.5, 1 and 1.5 g/L) were fabricated through cathodic electrophoretic deposition (EPD). The surface morphology and composition of the coatings revealed the formation of compact coatings with a uniform distribution of BG nanoparticles. Increasing the BG content enhanced the deposition rate of CS-BG nanocomposite coatings and raised the coating thickness. Moreover, the CS-BG coating containing 1.5 g/L BG showed the best corrosion performance owing to the more uniform distribution of BG nanoparticles and its higher thickness. Also, increasing the BG concentration improved the adhesion strength, raised the roughness, and promoted wettability. Further, in-vitro bioactivity evaluation of the coated and uncoated specimens in SBF revealed that the formation of bone-like apatite was significantly encouraged on the surface of CS-BG coatings, as compared to the Ti-6Al-4 V uncoated sample. So, the apatite-forming ability of the coatings was improved by increasing the BG content. For in vitro investigation, osteoblast-like cell line MG63 were cultured on Ti-6Al-4 V substrate coated with CS-BG and cellular behavior was evaluated. Results demonstrated good cell attachment with no significant levels of cytotoxicity during 5 days of culture. Therefore, the electrophoretic deposition of the CS-1.5 g/L BG coating could successfully enhance the adhesion strength, bioactivity, corrosion and cellular performance of the substrate.
壳聚糖-生物活性玻璃（CS-BG）纳米复合涂层被开发在 Ti-6Al-4V 合金上，以研究 BG 含量对结合强度、生物活性、生物腐蚀性、润湿性和粗糙度的影响。为此，采用溶胶-凝胶法合成了 BG 纳米粒子。通过阴极电泳沉积（EPD）制备了三种不同 BG 浓度（0.5、1 和 1.5g/L）的纳米复合涂层。涂层的表面形貌和组成表明形成了具有 BG 纳米粒子均匀分布的致密涂层。随着 BG 含量的增加，CS-BG 纳米复合涂层的沉积速率增加，涂层厚度增加。此外，含有 1.5g/L BG 的 CS-BG 涂层由于 BG 纳米粒子分布更均匀及其厚度更高，表现出最佳的耐腐蚀性。此外，随着 BG 浓度的增加，结合强度提高，粗糙度增加，润湿性提高。此外，在 SBF 中对涂层和未涂层试样的体外生物活性评估表明，与未涂层的 Ti-6Al-4V 试样相比，CS-BG 涂层表面明显促进了类骨磷灰石的形成。因此，通过增加 BG 含量提高了涂层的成骨能力。在体外研究中，将成骨样细胞系 MG63 培养在涂有 CS-BG 的 Ti-6Al-4V 基底上，并评估细胞行为。结果表明，在培养的 5 天内，细胞附着良好，无明显的细胞毒性。因此，CS-1.5g/L BG 涂层的电泳沉积可以成功提高基底的结合强度、生物活性、耐腐蚀性和细胞性能。
