Ye Xiaotong, Leeflang Sander, Wu Chengtie, Chang Jiang, Zhou Jie, Huan Zhiguang
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China.
University of Chinese Academy of Sciences, No.19(A), Yuquan Road, Shijingshan District, Beijing 100049, China.
Materials (Basel). 2017 Oct 27;10(11):1244. doi: 10.3390/ma10111244.
Porous Ti-6Al-4V scaffolds fabricated by means of selective laser melting (SLM), having controllable geometrical features and preferable mechanical properties, have been developed as a class of biomaterials that hold promising potential for bone repair. However, the inherent bio-inertness of the Ti-6Al-4V alloy as the matrix of the scaffolds results in a lack in the ability to stimulate bone ingrowth and regeneration. The aim of the present study was to develop a bioactive coating on the struts of SLM Ti-6Al-4V scaffolds in order to add the desired surface osteogenesis ability. Mesoporous bioactive glasses (MBGs) coating was applied on the strut surfaces of the SLM Ti-6Al-4V scaffolds through spin coating, followed by a heat treatment. It was found that the coating could maintain the characteristic mesoporous structure and chemical composition of MBG, and establish good interfacial adhesion to the Ti-6Al-4V substrate. The compressive strength and pore interconnectivity of the scaffolds were not affected by the coating. Moreover, the results obtained from in vitro cell culture experiments demonstrated that the attachment, proliferation, and differentiation of human bone marrow stromal cells (hBMSCs) on the MBG-coated Ti-6Al-4V scaffolds were improved as compared with those on the conventional bioactive glass (BG)-coated Ti-6Al-4V scaffolds and bare-metal Ti-6Al-4V scaffolds. Our results demonstrated that the MBG coating by using the spinning coating method could be an effective approach to achieving enhanced surface biofunctionalization for SLM Ti-6Al-4V scaffolds.
通过选择性激光熔化（SLM）制造的多孔Ti-6Al-4V支架，具有可控的几何特征和良好的机械性能，已被开发为一类在骨修复方面具有广阔前景的生物材料。然而，作为支架基体的Ti-6Al-4V合金固有的生物惰性导致其缺乏刺激骨向内生长和再生的能力。本研究的目的是在SLM Ti-6Al-4V支架的支柱上开发一种生物活性涂层，以增加所需的表面成骨能力。通过旋涂法将介孔生物活性玻璃（MBG）涂层施加到SLM Ti-6Al-4V支架的支柱表面，然后进行热处理。结果发现，该涂层可以保持MBG的特征介孔结构和化学成分，并与Ti-6Al-4V基体建立良好的界面附着力。支架的抗压强度和孔隙连通性不受涂层影响。此外，体外细胞培养实验结果表明，与传统生物活性玻璃（BG）涂层的Ti-6Al-4V支架和裸金属Ti-6Al-4V支架相比，人骨髓间充质干细胞（hBMSC）在MBG涂层的Ti-6Al-4V支架上的附着、增殖和分化得到了改善。我们的结果表明，采用旋涂法的MBG涂层可能是一种实现SLM Ti-6Al-4V支架表面生物功能增强的有效方法。
