College of Food and Biological Engineering, Jimei University, Xiamen, China.
Department of Bachelor Program of Biotechnology, National Chung Hsing University, Taichung City, Taiwan.
J Sci Food Agric. 2020 Jan 30;100(2):672-681. doi: 10.1002/jsfa.10063. Epub 2019 Nov 15.
Hibiscus sabdariffa is commonly used in daily life and its extract is applied widely in food and cosmetics. However, it has not been evaluated for its anti-aging effects.
Hibiscus sabdariffa calyx aqueous extract (HSCAE) has shown potential collagenase activity suppression effects, together with tyrosinase activity inhibition, and anti-oxidation as a free radical scavenger. The current investigation demonstrated that HSCAE was not cytotoxic in skin fibroblasts, and it significantly decreased ultraviolet B (UVB)-induced reactive oxygen species (ROS) on a flow cytometry assay. Moreover, HSCAE reduced matrix metalloproteinase (MMP) expression, increased tissue inhibition of metalloproteinase (TIMP)-1 level, and enhanced collagen content by inhibiting collagenase activity. It also blocked mRNA and protein expressions of melanin production pathway key factors, including the microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-1 (TRP-1), and dopachrome tautomerase-2 (TRP-2).
These results demonstrated, for the first time, the potential of HSCAE as a natural antioxidant with the ability to maintain collagen production and to decrease melanin syntheses under UVB radiation, for anti-aging effects. © 2019 Society of Chemical Industry.
洛神花在日常生活中被广泛使用，其提取物被广泛应用于食品和化妆品。然而，它的抗衰老作用尚未得到评估。
洛神花花萼水提物（HSCAE）具有潜在的胶原酶活性抑制作用，同时具有酪氨酸酶活性抑制作用和抗氧化作用，可作为自由基清除剂。目前的研究表明，HSCAE 在皮肤成纤维细胞中无细胞毒性，并且在流式细胞术分析中显著降低了紫外线 B（UVB）诱导的活性氧（ROS）。此外，HSCAE 通过抑制胶原酶活性，减少基质金属蛋白酶（MMP）的表达，增加组织金属蛋白酶抑制剂（TIMP）-1 水平，并增强胶原蛋白含量。它还阻断了黑色素生成途径关键因子的 mRNA 和蛋白表达，包括小眼畸形相关转录因子（MITF）、酪氨酸酶、酪氨酸酶相关蛋白-1（TRP-1）和多巴色素互变异构酶-2（TRP-2）。
这些结果首次表明，HSCAE 作为一种天然抗氧化剂，具有在 UVB 辐射下维持胶原蛋白生成和减少黑色素合成的潜力，可达到抗衰老的效果。© 2019 英国化学学会。
