Satti Parvathi, Kakarla Pranitha, Jogendra Avula Sai Sankar, Muppa Radhika, Kiran Rompicharla Sri Vishnu, Biswas Swati
Department of Pedodontics and Preventive Dentistry, Sibar Institute of Dental Sciences, Guntur, Andhra Pradesh, India.
Department of Pedodontics and Preventive Dentistry, Drs. Sudha and Nageshwar Rao Siddhartha Institute of Dental Sciences, Krishna, Andhra Pradesh, India.
J Indian Soc Pedod Prev Dent. 2019 Jul-Sep;37(3):275-281. doi: 10.4103/JISPPD.JISPPD_112_19.
The success of an endodontic treatment depends on effective disinfection and complete sealing of root canal. The irrigants that are currently used in the field of endodontics have certain limitations, so the quest for an ideal root canal irrigant continues. Nowadays, the use of herbal extracts such as Triphala and liquorice are used for their potent antimicrobial activity and less side effects.
This study aims (1) to evaluate the antimicrobial efficacy of Triphala and liquorice against Enterococcus faecalis.(2) To determine any cytotoxic effect on isolated human periodontal ligament (PDL) fibroblasts.
The antimicrobial efficacy of Triphala and liquorice extracts was analyzed at different concentrations (12.5, 25, 50, 75, and 100 mg/ml) using the well-diffusion method. Three percent sodium hypochlorite (NaOCl) and distilled water were taken as positive and negative controls. Minimum inhibitory concentration of the active extract was determined by the broth dilution assay. Human PDL fibroblast tissue culture was used to assess the cytotoxicity of the preparations. The data thus obtained were subjected to statistical analysis.
The results showed that the mean antimicrobial efficacy of Triphala and liquorice at 50 mg/ml is 20.33 and 9.33, respectively, which are statistically significant (P < 0.0001) as compared with a concentration 12.5 and 25 mg/ml. 50 mg/ml showed significant results (P < 0.001) on comparing with hypochlorite. Triphala and liquorice showed no cytotoxic effect as compared to NaOCl on human PDL fibroblasts.
Among the three tested materials Triphala showed the highest antimicrobial efficacy followed by NaOCl and liquorice.
根管治疗的成功取决于根管的有效消毒和完全封闭。目前牙髓病学领域使用的冲洗剂有一定局限性，因此对理想根管冲洗剂的探索仍在继续。如今，诸如三果木和甘草等草药提取物因其强大的抗菌活性和较少的副作用而被使用。
本研究旨在（1）评估三果木和甘草对粪肠球菌的抗菌效果。（2）确定对分离的人牙周膜（PDL）成纤维细胞的任何细胞毒性作用。
采用琼脂扩散法分析不同浓度（12.5、25、50、75和100mg/ml）的三果木和甘草提取物的抗菌效果。以3%次氯酸钠（NaOCl）和蒸馏水作为阳性和阴性对照。通过肉汤稀释法测定活性提取物的最低抑菌浓度。用人PDL成纤维细胞组织培养评估制剂的细胞毒性。对由此获得的数据进行统计分析。
结果显示，三果木和甘草在50mg/ml时的平均抗菌效果分别为20.33和9.33，与12.5mg/ml和25mg/ml的浓度相比具有统计学意义（P<0.0001）。与次氯酸钠相比，50mg/ml显示出显著结果（P<0.001）。与NaOCl相比，三果木和甘草对人PDL成纤维细胞没有细胞毒性作用。
在三种测试材料中，三果木显示出最高的抗菌效果，其次是NaOCl和甘草。
