Amity Center for Nanobiotechnology and Nanomedicine (ACNN), Amity Institute of Biotechnology, Amity University Rajasthan, Kant Kalwar, NH-11C, Jaipur-Delhi Highway, Jaipur, 303002, Rajasthan, India.
Nano-Bio Interfacial Research Laboratory (NBIRL), Department of Biotechnology, Siddaganga Institute of Technology, Tumkur, 572103, Karnataka, India; Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode - 638401, Tamil Nadu, India.
Colloids Surf B Biointerfaces. 2019 Dec 1;184:110522. doi: 10.1016/j.colsurfb.2019.110522. Epub 2019 Sep 25.
With the rapidly approaching post-antibiotic era, new and effective combinations of antibiotics are imperative to combat multiple drug resistance (MDR). We have synthesized multimodal antimicrobials that integrate the antibiotic isonicotinylhydrazide (INH), silver nanoparticles (AgNPs), and two different polyoxometalates (POMs) namely, phosphotungstic acid (PTA) and phosphomolybdic acid (PMA) to prepare AgNPs and AgNPs, respectively. AgNPs have peroxidase-like (nanozyme) activity and very high antibacterial potential toward S. aureus, which was further enhanced upon modification with POMs. The selectivity of these functional nanozymes was evaluated with m5S mouse fibroblasts using WST-8, LDH viability, in vitro reactive oxygen species (ROS) generation assays, and crystal violet morphological studies. These investigations showed very low cytotoxicity for the nanoparticles compared to free metal ions (Ag), pristine POMs and INH, demonstrating the ability of multifunctional materials to provide efficient and selective antimicrobials.
随着后抗生素时代的迅速临近，需要将新的、有效的抗生素组合来对抗多种药物耐药性（MDR）。我们合成了多模式抗菌药物，将抗生素异烟肼酰肼（INH）、银纳米粒子（AgNPs）和两种不同的多金属氧酸盐（POMs）即磷钨酸（PTA）和磷钼酸（PMA）整合在一起，分别制备 AgNPs 和 AgNPs。AgNPs 具有过氧化物酶样（纳米酶）活性，对金黄色葡萄球菌具有很高的抗菌潜力，而经过 POMs 修饰后，其抗菌潜力进一步增强。使用 WST-8、LDH 活力、体外活性氧（ROS）生成测定和结晶紫形态学研究，用 m5S 小鼠成纤维细胞评估了这些功能纳米酶的选择性。与游离金属离子（Ag）、原始 POMs 和 INH 相比，这些纳米颗粒的细胞毒性非常低，证明了多功能材料具有提供高效和选择性抗菌药物的能力。
