Shinohara Yuta, Oyama Ayaka, Usui Tatsuya, Sasaki Kazuaki
Laboratory of Veterinary Pharmacology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Pet Health & Food Division, Iskara Industry Co., Ltd., 1-14-2, Nihonbashi, Chuo-ku, Tokyo 103-0027, Japan.
J Vet Med Sci. 2019 Nov 29;81(11):1616-1620. doi: 10.1292/jvms.19-0380. Epub 2019 Oct 4.
It is known that oxidative stress is related to disease in humans and dogs. Many traditional Chinese medicines have been reported to have anti-oxidative effects, but there are no reports that they have anti-oxidative effects in dogs. In this study, we examined the anti-oxidative effects of Juzen-taiho-to, a traditional Chinese medicine, in dogs. Five healthy female beagle dogs (38-41 months of age weighing 8.6-10.7 kg) were orally administered Juzen-taiho-to at 450 mg/kg with food for 28 days. Blood samples were taken from all five dogs on days 0, 7, 14, 21, and 28. Using the blood samples, improvement of the antioxidant level as assessed by the biological antioxidant potential (BAP), reduced oxidative stress level as assessed by derivatives of reactive oxygen metabolites (d-ROMs), and improvement of blood fluidity were examined. Regarding the antioxidant level and blood fluidity, no significant difference was observed, but the oxidative stress level on days 14, 21, and 28 was significantly lower than that on day 0. Thus, Juzen-taiho-to may have anti-oxidative effects in dogs by reducing oxidative stress and be useful for oxidative stress-related diseases in dogs.
已知氧化应激与人类和犬类的疾病有关。许多中药已被报道具有抗氧化作用，但尚无它们对犬类具有抗氧化作用的报道。在本研究中，我们检测了中药十全大补汤对犬类的抗氧化作用。选取5只健康雌性比格犬（年龄38 - 41个月，体重8.6 - 10.7千克），将十全大补汤以450毫克/千克的剂量与食物一起口服给药28天。在第0、7、14、21和28天从所有5只犬采集血样。利用这些血样，检测了通过生物抗氧化能力（BAP）评估的抗氧化水平的改善情况、通过活性氧代谢产物衍生物（d-ROMs）评估的氧化应激水平的降低情况以及血液流动性的改善情况。关于抗氧化水平和血液流动性，未观察到显著差异，但第14、21和28天的氧化应激水平显著低于第0天。因此，十全大补汤可能通过降低氧化应激对犬类具有抗氧化作用，并且对犬类与氧化应激相关的疾病可能有用。
