Department of Dentistry, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Fuzhou, 350005, China.
Department of Preventive Dentistry, School and Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Middle Road, Fuzhou 350000, China.
Int J Biol Sci. 2019 Aug 24;15(11):2330-2339. doi: 10.7150/ijbs.33881. eCollection 2019.
Many studies have shown that FZD2 is significantly associated with tumor development and tumor metastasis. The purpose of the present study was to gain insight into the role of FZD2 in the cell proliferation and invasion of tongue squamous cell carcinoma. According to TCGA-HNSC dataset, among the 10 Frizzled receptors, FZD2 exhibited the highest degree of differential expression between cancer tissues and normal tissues, and the overall survival of patients with higher FZD2 levels was shown to be significantly shorter compared with those with lower FZD2 levels. The upregulation of FZD2 in clinical tongue cancer tissues was validated by real-time PCR. Knockdown of FZD2 inhibited the proliferation, migration and invasion of CAL-27 and TCA-8113 cells, whereas overexpression of FZD2 led to the opposite results. Further analysis revealed that FZD2 is positively correlated with WNT3A, WNT5B, WNT7A and WNT2 and is negatively correlated with WNT4. These results indicated that FZD2 may act as an oncogene in tongue squamous cell carcinoma. Therefore, FZD2 may be a target for the diagnosis, prognosis and gene therapy of tongue cancer.
许多研究表明，FZD2 与肿瘤的发生和转移显著相关。本研究旨在深入了解 FZD2 在舌鳞癌细胞增殖和侵袭中的作用。根据 TCGA-HNSC 数据集，在 10 个卷曲受体中，FZD2 在癌组织和正常组织之间的差异表达程度最高，并且 FZD2 水平较高的患者的总体生存率明显短于 FZD2 水平较低的患者。实时 PCR 验证了 FZD2 在临床舌癌组织中的上调。FZD2 的敲低抑制了 CAL-27 和 TCA-8113 细胞的增殖、迁移和侵袭，而过表达 FZD2 则导致相反的结果。进一步分析表明，FZD2 与 WNT3A、WNT5B、WNT7A 和 WNT2 呈正相关，与 WNT4 呈负相关。这些结果表明，FZD2 可能在舌鳞癌细胞癌中作为癌基因发挥作用。因此，FZD2 可能是舌癌的诊断、预后和基因治疗的靶点。
