Sparticolins A-G, Biologically Active Oxidized Spirodioxynaphthalene Derivatives from the Ascomycete .

To explore the chemical diversity of metabolites from new species of Dothideomycetes, the ex-type strain of was investigated. Seven highly oxygenated and functionalized spirodioxynaphthalene natural products incorporating carboxyalkylidene-cyclopentanoid (-), carboxyl-functionalized oxabicyclo[3.3.0]octane (-), and annelated 2-cyclopentenone/δ-lactone () units, sparticolins A-G, were isolated from submerged cultures of the fungus. Their chemical structures including their relative (and absolute) configurations were established through spectroscopic and X-ray crystallographic analyses. Sparticolin B () exhibited inhibitory activity against the Gram-positive bacteria , , and , while sparticolin G () showed antifungal activities against and . All other sparticolins were only weakly active against and also showed weak activities against the nematode . Compounds and also showed moderate cytotoxic activities against seven mammalian cell lines.