γ-谷氨酰循环利用细胞内谷胱甘肽在体内发挥作用的证据：氨基酸的影响及酶的选择性抑制
Evidence that the gamma-glutamyl cycle functions in vivo using intracellular glutathione: effects of amino acids and selective inhibition of enzymes.
作者信息
Griffith O W, Bridges R J, Meister A
出版信息
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5405-8. doi: 10.1073/pnas.75.11.5405.
Abstract
The function of the gamma-glutamyl cycle was explored in in vivo studies in which amino acids and specific inhibitors of cycle enzymes (gamma-glutamyl transpeptidase, gamma-glutamyl cyclotransferase, gamma-glutamylcysteine synthetase, and 5-oxoprolinase) were administered to mice. The findings, which show that the gamma-glutamyl cycle functions in vivo, support the conclusion that gamma-glutamyl amino acids formed by gamma-glutamyl transpeptidase from externally supplied amino acids and intracellular glutathione are translocated into the cell and thus indicate that there is a significant physiological connection between the metabolism of glutathione and the transport of amino acids.
摘要
在体内研究中探索了γ-谷氨酰循环的功能，该研究向小鼠施用了氨基酸和循环酶（γ-谷氨酰转肽酶、γ-谷氨酰环化转移酶、γ-谷氨酰半胱氨酸合成酶和5-氧脯氨酸酶）的特异性抑制剂。研究结果表明γ-谷氨酰循环在体内发挥作用，支持了以下结论：由γ-谷氨酰转肽酶从外部供应的氨基酸和细胞内谷胱甘肽形成的γ-谷氨酰氨基酸被转运到细胞内，因此表明谷胱甘肽代谢与氨基酸转运之间存在重要的生理联系。
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本文引用的文献
1
The reaction of glutathione with amino acids and related compounds as catalyzed by gamma-glutamyl transpeptidase.γ-谷氨酰转肽酶催化的谷胱甘肽与氨基酸及相关化合物的反应。
J Biol Chem. 1959 Mar;234(3):577-82.
2
Inhibition of 5-oxoprolinase by 2-imidazolidone-4-carboxylic acid.2-咪唑烷酮-4-羧酸对5-氧代脯氨酸酶的抑制作用。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):759-61. doi: 10.1073/pnas.70.3.759.
3
Interaction of gamma-glutamyl transpeptidase with amino acids, dipeptides, and derivatives and analogs of glutathione.γ-谷氨酰转肽酶与氨基酸、二肽以及谷胱甘肽的衍生物和类似物的相互作用。
J Biol Chem. 1974 Dec 10;249(23):7593-602.
4
Glutathione turnover in the kidney; considerations relating to the gamma-glutamyl cycle and the transport of amino acids.肾脏中的谷胱甘肽周转；与γ-谷氨酰循环及氨基酸转运相关的考量
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2969-72. doi: 10.1073/pnas.71.8.2969.
5
Glutathione synthetase deficiency, an inborn error of metabolism involving the gamma-glutamyl cycle in patients with 5-oxoprolinuria (pyroglutamic aciduria).谷胱甘肽合成酶缺乏症，一种涉及5-氧脯氨酸尿症（焦谷氨酸尿症）患者γ-谷氨酰循环的先天性代谢缺陷。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2505-9. doi: 10.1073/pnas.71.6.2505.
6
Accumulation of 5-oxoproline in mouse tissues after inhibition of 5-oxoprolinase and administration of amino acids: evidence for function of the gamma-glutamyl cycle.5-氧脯氨酸酶抑制及氨基酸给药后小鼠组织中5-氧脯氨酸的积累：γ-谷氨酰循环功能的证据
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1026-9. doi: 10.1073/pnas.71.4.1026.
7
On the enzymology of amino acid transport.论氨基酸转运的酶学
Science. 1973 Apr 6;180(4081):33-9. doi: 10.1126/science.180.4081.33.
8
Subcellular localization of rat kidney phosphate independent glutaminase.大鼠肾磷酸非依赖性谷氨酰胺酶的亚细胞定位
Arch Biochem Biophys. 1975 Apr;167(2):519-24. doi: 10.1016/0003-9861(75)90494-4.
9
Decrease in glutathione levels of kidney and liver after injection of methionine sulfoximine into rats.向大鼠注射蛋氨酸亚砜亚胺后肾脏和肝脏中谷胱甘肽水平的降低。
Biochem Biophys Res Commun. 1975 Feb 3;62(3):651-7. doi: 10.1016/0006-291x(75)90448-9.
10
Synthesis of ophthalmic acid in liver and kidney in vivo.体内肝脏和肾脏中眼酸的合成。
Biochem J. 1978 Feb 15;170(2):415-9. doi: 10.1042/bj1700415.
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