颞下颌关节骨关节炎滑膜组织中环状RNA的表达谱及具有特定反向剪接连接的hsa_circ_0000448的潜在功能
Expression profile of circular RNA s in TMJ osteoarthritis synovial tissues and potential functions of hsa_circ_0000448 with specific back-spliced junction.
作者信息
Hu Yihui, Zhu Huimin, Bu Lingtong, He Dongmei
机构信息
Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine Shanghai 200011, China.
出版信息
Am J Transl Res. 2019 Sep 15;11(9):5357-5374. eCollection 2019.
OBJECTIVE
As essential players in the occurrence and development of osteoarthritis, circular RNAs (circRNAs) have gradually received attention in recent years. However, their roles in temporomandibular joint osteoarthritis (TMJOA) featured with pain, restricted mouth opening and joint movement dysfunction, remains elusive.
METHODS
The expression profile of circRNAs in TMJ synovial tissues was analyzed by RNA-Seq. The differentially expressed circRNAs in the TMJOA were identified. The potential biological functions of these circRNAs were evaluated.
RESULTS
The expression profile of circRNAs in TMJ synovial tissues was stable and abundant, and most of which were newly discovered. A total of 58 differentially expressed circRNAs were identified in TMJOA, and four of which were identified by experiments. Among them, the up-regulated hsa_circ_0000448 with specific back-splice junction was involved in the TNF-α signaling pathway through CeRNA mechanism by targeting related microRNAs. Additionally, it was also predicted to bind several RNA binding proteins (RBPs), but almost had no protein-coding ability.
CONCLUSION
circRNAs in TMJ synovial tissue participate in the progression of TMJOA and may become a potential therapeutic target. The highly up-regulated has_circ_0000448 probably promotes TNF-α secretion of synovium through CeRNA mechanism in TMJOA.
目的
作为骨关节炎发生发展的关键参与者，环状RNA（circRNAs）近年来逐渐受到关注。然而，它们在以疼痛、张口受限和关节运动功能障碍为特征的颞下颌关节骨关节炎（TMJOA）中的作用仍不清楚。
方法
通过RNA测序分析颞下颌关节滑膜组织中circRNAs的表达谱。鉴定TMJOA中差异表达的circRNAs。评估这些circRNAs的潜在生物学功能。
结果
颞下颌关节滑膜组织中circRNAs的表达谱稳定且丰富，其中大部分是新发现的。在TMJOA中总共鉴定出58个差异表达的circRNAs，其中4个通过实验得到验证。其中，具有特定反向剪接连接的上调的hsa_circ_0000448通过靶向相关微小RNA，通过竞争性内源RNA（CeRNA）机制参与肿瘤坏死因子-α（TNF-α）信号通路。此外，它还被预测可结合几种RNA结合蛋白（RBPs），但几乎没有蛋白质编码能力。
结论
颞下颌关节滑膜组织中的circRNAs参与TMJOA的进展，可能成为潜在的治疗靶点。高度上调的has_circ_0000448可能通过CeRNA机制促进TMJOA中滑膜TNF-α的分泌。
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