Dermatologic Clinic, Taiwan.
Int Wound J. 2020 Feb;17(1):158-166. doi: 10.1111/iwj.13250. Epub 2019 Oct 29.
Treating chronic skin wounds in patients with diabetes, bed sores, or stasis dermatitis is typically a time-consuming and costly process, and the outcome is not always promising. Concentrated growth factor (CGF) obtained from the autologous venous blood of patients via fractional centrifugation is employed for producing a CGF gel or membrane that can be applied to expedite self-regeneration of skin wounds. In this case report, we presented the results from 18 patients with chronic skin wounds treated with a CGF gel or membrane produced from autologous venous blood. Noticeable granulation tissue and regenerated epidermal coverage were observed in 16 patients who received CGF treatment over various time courses, thereby demonstrating the significant therapeutic effects of CGF treatment in overall wound healing. The other two patients with stasis ulcers in their calves failed to respond to the treatment because of the comorbidity of iliac vein thrombosis. In addition, by culturing HaCaT keratinocytes using CGF membrane as the foundation, we observed that HaCaT cells attached to the CGF membrane migrated and proliferated to form an epithelium-like structure. Comprehensively, the clinical results infer that CGF gel can expedite the regeneration of the soft tissue at the wound, whereas CGF membrane may facilitate its marginal re-epithelialisation. The combination of the two can promote autologous regeneration of both deep and superficial wounds effectively and safely.
治疗糖尿病患者的慢性皮肤伤口、褥疮或淤滞性皮炎通常是一个耗时且昂贵的过程，而且结果并不总是有希望的。通过分阶段离心从患者自身静脉血中获得的浓缩生长因子（CGF）用于产生 CGF 凝胶或膜，可加速皮肤伤口的自我再生。在本病例报告中，我们介绍了 18 例使用从自体静脉血制备的 CGF 凝胶或膜治疗慢性皮肤伤口的结果。在不同的时间过程中，16 例接受 CGF 治疗的患者观察到明显的肉芽组织和再生的表皮覆盖，从而证明了 CGF 治疗在整体伤口愈合中的显著治疗效果。另外两名小腿淤滞性溃疡患者由于髂静脉血栓形成的合并症对治疗没有反应。此外，通过使用 CGF 膜作为基础培养 HaCaT 角质形成细胞，我们观察到 HaCaT 细胞附着在 CGF 膜上迁移和增殖，形成上皮样结构。综上所述，临床结果推断 CGF 凝胶可以加速伤口处软组织的再生，而 CGF 膜可能有助于其边缘再上皮化。两者的结合可以有效和安全地促进深部和浅部伤口的自体再生。
