Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, 00970, Palestine.
Department of Chemistry, Faculty of Science, An-Najah National University, Nablus, 00970, Palestine.
Molecules. 2019 Oct 28;24(21):3880. doi: 10.3390/molecules24213880.
The present study aimed to identify the chemical constituents and to assess the in-vitro, antimicrobial, anticancer, antioxidant, metabolic enzymes and cyclooxygenase (COX) inhibitory properties of essential oil (EO) of Boiss. leaves. The EO was isolated and identified using microwave-ultrasonic and GC-MS techniques, respectively. Fifty-two compounds were identified, of which -borneol was the major component, followed by eucalyptol and epizonarene. The EO was evaluated against a panel of in-vitro bioassays. The EO displayed antimicrobial activity against methicillin-resistant (MRSA), and , with MIC values of 0.039, 0.078 and 0.78 mg/mL, respectively. The EO exhibited cytotoxicity against HeLa (cervical adenocarcinoma) and Colo-205 (colon) cancer cell lines with percentages of inhibition of 95% and 90%, for EO concentrations of 1.25 and 0.5 mg/mL, respectively. Furthermore, it showed metabolic enzyme (α-amylase, α-glucosidase, and lipase) inhibitory (IC = 45.22 ± 1.1, 63.09 ± 0.26, 501.18 ± 0.38 µg/mL, respectively) and antioxidant activity, with an IC value of 19.95 ± 2.08 µg/mL. Moreover, the EO showed high cyclooxygenase inhibitory activity against COX-1 and COX-2 with IC values of 0.25 and 0.5 µg/mL, respectively, similar to those of the positive control (the NSAID etodolac). Outcomes amassed from this investigation illustrate that EO represents a rich source of pharmacologically active molecules which can be further validated and explored clinically for its therapeutic potential and for the development and design of new natural therapeutic preparations.
本研究旨在鉴定 Boiss. 叶精油的化学成分，并评估其体外抗菌、抗癌、抗氧化、代谢酶和环氧化酶（COX）抑制活性。精油采用微波超声和 GC-MS 技术分别进行分离和鉴定。共鉴定出 52 种化合物，其中 - 龙脑为主要成分，其次为桉油醇和 epizonarene。该精油对一系列体外生物测定进行了评估。精油对耐甲氧西林金黄色葡萄球菌（MRSA）和 具有抗菌活性，MIC 值分别为 0.039、0.078 和 0.78 mg/mL。精油对 HeLa（宫颈腺癌）和 Colo-205（结肠）癌细胞系表现出细胞毒性，当精油浓度分别为 1.25 和 0.5 mg/mL 时，抑制率分别为 95%和 90%。此外，它还表现出代谢酶（α-淀粉酶、α-葡萄糖苷酶和脂肪酶）抑制作用（IC = 45.22 ± 1.1、63.09 ± 0.26、501.18 ± 0.38 µg/mL）和抗氧化活性，IC 值为 19.95 ± 2.08 µg/mL。此外，该精油对 COX-1 和 COX-2 具有高的环氧化酶抑制活性，IC 值分别为 0.25 和 0.5 µg/mL，与阳性对照（非甾体抗炎药依托度酸）相似。本研究的结果表明，精油是具有药理活性的分子的丰富来源，可以进一步验证和探索其在治疗潜力方面的临床应用，以及用于开发和设计新的天然治疗制剂。
