Shehadeh Mayadah, Jaradat Nidal, Al-Masri Motasem, Zaid Abdel Naser, Hussein Fatima, Khasati Ahmad, Suaifan Ghadeer, Darwish Rula
Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman 11942, Jordan.
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, P.O. Box 7, Nablus, Palestinian Authority.
Saudi Pharm J. 2019 Jul;27(5):612-618. doi: 10.1016/j.jsps.2019.03.001. Epub 2019 Mar 2.
() is a very popular edible and medicinal plant in the East Mediterranean countries. The aims of the current study were to use microwave-ultrasonic assisted hydrodistillation (MUAHD) method to produce essential oils (EOs) from wild samples collected from four different geographical areas in The West Bank using water as a solvent, determine the phytochemical profile using GC-MS analysis and assess their antioxidant and antibacterial potential.
Essential oils were produced using MUAHD method. Gas chromatography coupled with mass spectrometer detector (GC-MS) was employed for phytochemical analysis. antibacterial and antioxidant potentials were carried out.
Differences in the EOs yield among the four samples were observed. GC-MS analysis of EOs revealed terpenes as the major constituents; monoterpenes (22-56%) and oxygenated monoterpenes (28-57%). Thymol, α-terpinene and carvacrol represent the bulk of all phytochemicals detected by GC-MS analysis. γ-Terpinene-rich EOs, exhibited the highest antioxidant capacity. Thymol-rich EOs were found to be most effective against and Methicillin-Resistant (MRSA) (MIC 390 µg/mL). Alpha-terpinene-rich chemotype EOs exhibited the highest inhibitory effect of (MIC of 1560 µg/mL). Interestingly, γ-terpinene-rich EO showed promising antibacterial properties against (MIC 97 µg/mL) and a powerful anti-oxidant effect (91.45% ±2.30).
The current study supports the use of MUAHD as a time-saving, cost-effective, environment-friendly method for production of high quality EO for potential use as a natural complementary treatment and in the prevention of bacterial infections as well as oxidation by free radicals without compromising the quantity.
（）是东地中海国家一种非常受欢迎的食用和药用植物。本研究的目的是使用微波-超声辅助水蒸馏（MUAHD）方法，以水为溶剂，从约旦河西岸四个不同地理区域采集的野生样本中提取精油（EOs），通过气相色谱-质谱联用（GC-MS）分析确定其植物化学特征，并评估其抗氧化和抗菌潜力。
采用MUAHD方法生产精油。使用气相色谱-质谱联用仪（GC-MS）进行植物化学分析，并进行抗菌和抗氧化潜力测试。
观察到四个样本的精油产量存在差异。GC-MS分析表明，萜类化合物是精油的主要成分；单萜（22-56%）和氧化单萜（28-57%）。百里香酚、α-萜品烯和香芹酚是GC-MS分析检测到的所有植物化学物质的主要成分。富含γ-萜品烯的精油表现出最高的抗氧化能力。发现富含百里香酚的精油对（）和耐甲氧西林金黄色葡萄球菌（MRSA）最有效（最低抑菌浓度为390μg/mL）。富含α-萜品烯化学型的精油对（）表现出最高的抑制作用（最低抑菌浓度为1560μg/mL）。有趣的是，富含γ-萜品烯的精油对（）显示出有前景的抗菌特性（最低抑菌浓度为97μg/mL）和强大的抗氧化作用（91.45%±2.30）。
本研究支持使用MUAHD作为一种省时、经济高效、环境友好的方法来生产高质量的（）精油，该精油有潜力用作天然辅助治疗药物，用于预防细菌感染以及自由基氧化，且不影响产量。
