Al-Maharik Nawaf, Jaradat Nidal, Qneibi Mohammad, Abualhasan Murad N, Emwas Nour
Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus, State of Palestine.
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus, State of Palestine.
Evid Based Complement Alternat Med. 2020 Nov 23;2020:4195272. doi: 10.1155/2020/4195272. eCollection 2020.
The rise of the emergence of microbial resistance of antibiotics, the dangerous side effects of nonsteroidal anti-inflammatory drugs, and noncompetent medications of Alzheimer's, Parkinson's, and other neurodegenerative diseases prompt scientists to search for phytochemicals that could be utilized in the remedy of lethal diseases. (Boiss.) Kuntze () is a medicinal herb growing in the eastern parts of the Mediterranean Sea Basin and is widely consumed as a tea. The leaves of this plant have been traditionally used for the treatment of various infectious diseases. The current research was designed to identify the chemical composition of (Boiss.) essential oil (EO) and to assess its antibacterial, antifungal, and cyclooxygenase inhibitory activities and the biophysical gating effect on AMPA receptors. Twenty phytochemicals were identified from leaves and flowers EO amounting to almost 100% of the total constituents using GC-MS technique, of which 1,6-dimethylspiro[4.5]decane (27.51%) , caryophyllene oxide (20.08%) , and -caryophyllene (18.28%) were the main constituents. The biophysical properties' effect from the plant extract on various AMPA-type receptors expressed in Human Embryonic Kidney (HEK293) cells was assessed by exploiting the whole-cell patch-clamp technique. Microdilution assay was adopted for assessing the antimicrobial property against eight virulent microbial strains whilst the cyclooxygenase inhibition effect was accomplished utilizing COX inhibitory screening colorimetric assay EO displayed potent activity against (MIC = 1.25 g/mL), (MIC = 3.12 g/mL), and (MIC = 1.25 g/mL), compared with ciprofloxacin (positive control) and potent antibacterial activity against , MRSA, , , and compared to Ampicillin (2nd positive control). It also showed anti- (MIC = 6.25 g/mL) and antimold (MIC = 3.125 g/mL) activities compared with fluconazole (antifungal positive control). Likewise, our results showed an inhibition and biophysical impact of . curviflora on all AMPARs subunits.
抗生素微生物耐药性的不断出现、非甾体抗炎药的危险副作用以及用于治疗阿尔茨海默病、帕金森病和其他神经退行性疾病的药物疗效不佳，促使科学家们寻找可用于治疗致命疾病的植物化学物质。(Boiss.) Kuntze是一种生长在地中海盆地东部的药草，人们广泛将其作为茶饮用。这种植物的叶子传统上用于治疗各种传染病。当前的研究旨在确定(Boiss.)精油（EO）的化学成分，并评估其抗菌、抗真菌和环氧化酶抑制活性以及对AMPA受体的生物物理门控作用。使用气相色谱 - 质谱联用（GC - MS）技术从叶子和花朵的EO中鉴定出20种植物化学物质，其含量几乎占总成分的100%，其中1,6 - 二甲基螺[4.5]癸烷（27.51%）、氧化石竹烯（20.08%）和β - 石竹烯（18.28%）是主要成分。通过全细胞膜片钳技术评估了植物提取物对人胚肾（HEK293）细胞中表达的各种AMPA型受体的生物物理特性影响。采用微量稀释法评估对八种强毒微生物菌株的抗菌性能，同时利用COX抑制筛选比色法完成环氧化酶抑制作用评估。与环丙沙星（阳性对照）相比，EO对大肠杆菌（MIC = 1.25μg/mL）、金黄色葡萄球菌（MIC = 3.12μg/mL）和白色念珠菌（MIC = 1.25μg/mL）显示出强效活性，与氨苄青霉素（第二阳性对照）相比，对肺炎克雷伯菌、耐甲氧西林金黄色葡萄球菌、铜绿假单胞菌、鲍曼不动杆菌和粪肠球菌显示出强效抗菌活性。与氟康唑（抗真菌阳性对照）相比，它还显示出抗白色念珠菌（MIC = 6.25μg/mL）和抗霉菌（MIC = 3.125μg/mL）活性。同样，我们的结果显示了弯花筋骨草对所有AMPA受体亚基的抑制作用和生物物理影响。
