Menin Barbara, Lami Andrea, Musazzi Simona, Petrova Anastasia A, Santabarbara Stefano, Casazza Anna Paola
Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Via Bassini 15a, 20133 Milano, Italy.
Photosynthesis Research Unit, Consiglio Nazionale delle Ricerche, Via Celoria 26, 20133 Milano, Italy.
Microorganisms. 2019 Oct 28;7(11):501. doi: 10.3390/microorganisms7110501.
The model cyanobacterium sp. PCC 6803 has gained significant attention as an alternative and sustainable source for biomass, biofuels and added-value compounds. The latter category includes keto-carotenoids, which are molecules largely employed in a wide spectrum of industrial applications in the food, feed, nutraceutical, cosmetic and pharmaceutical sectors. Keto-carotenoids are not naturally synthesized by , at least in any significant amounts, but their accumulation can be induced by metabolic engineering of the endogenous carotenoid biosynthetic pathway. In this study, the accumulation of the keto-carotenoids astaxanthin and canthaxanthin, resulting from the constitutive or temperature-inducible expression of the and genes from , is compared. The benefits and drawbacks of the two engineering approaches are discussed.
模式蓝细菌集胞藻PCC 6803作为生物质、生物燃料和增值化合物的替代且可持续来源已受到广泛关注。后一类包括酮类胡萝卜素，这类分子广泛应用于食品、饲料、营养保健品、化妆品和制药等众多工业领域。酮类胡萝卜素并非集胞藻PCC 6803自然合成的，至少不会大量合成，但其积累可通过对内源类胡萝卜素生物合成途径进行代谢工程来诱导。在本研究中，比较了由来自欧文氏菌的crtW和crtZ基因组成型或温度诱导型表达所导致的酮类胡萝卜素虾青素和角黄素的积累情况。并讨论了这两种工程方法的优缺点。
