Department of Otolaryngology and Laryngeal Oncology, Medical University of Lublin, Lublin, Poland.
Department of Clinical Immunology and Immunotherapy, Medical University of Lublin, Lublin, Poland.
In Vivo. 2019 Nov-Dec;33(6):2007-2012. doi: 10.21873/invivo.11697.
BACKGROUND/AIM: Chronic viral infection is an important risk factor in the development of cancer. Failure of immune response to clear the oncogenic infection can facilitate cancer progression. The aim of the present study was to analyze early and late activation of T-lymphocytes related to Epstein-Barr virus (EBV) infection by the expression of markers of activation (CD69, CD25) on the surface of T-lymphocytes (CD3, CD4, CD8) in patients bearing laryngeal cancer according to absence/presence immunoglobulin G antibodies to EBV nuclear antigen (EBNA1).
Thirty-three patients with laryngeal squamous cell carcinoma (LC) and 20 volunteers without cancer (control group) were enrolled in the study. Peripheral blood samples were collected from every individual. The markers of activation of T-lymphocytes were determined by flow cytometry, whereas commercial immunoenzymatic assay kits were used for detection of anti-viral capsid antigen (VCA) IgM, anti-VCA IgG, and anti-EBNA1 IgG.
Increased early activation of CD8 and CD4 T-lymphocytes was found in patients with LC. There was a significantly higher proportion of CD4 and CD8T-lymphocytes expressing CD69 antigen in patients with LC compared to the control group. The proportion of CD4 CD25 T-lymphocytes in patients with LC positive for anti-EBNA1 IgG and anti-VCA IgM was lower compared to patients without antibodies to VCA IgM.
The dysfunction of immune response in larynx cancer patients could be associated with EBV infection.
背景/目的：慢性病毒感染是癌症发展的一个重要危险因素。免疫反应未能清除致癌感染可促进癌症进展。本研究旨在通过分析与 Epstein-Barr 病毒（EBV）感染相关的 T 淋巴细胞的早期和晚期激活，来研究表达 T 淋巴细胞（CD3、CD4、CD8）表面的激活标志物（CD69、CD25）的喉癌患者中，根据是否存在针对 EBV 核抗原（EBNA1）的免疫球蛋白 G 抗体，来分析 T 淋巴细胞的早期和晚期激活。
本研究纳入了 33 例喉鳞状细胞癌（LC）患者和 20 名无癌症的志愿者（对照组）。从每个人采集外周血样本。通过流式细胞术测定 T 淋巴细胞的激活标志物，同时使用商业酶联免疫吸附试验试剂盒检测抗病毒衣壳抗原（VCA）IgM、抗-VCA IgG 和抗-EBNA1 IgG。
在 LC 患者中发现 CD8 和 CD4 T 淋巴细胞的早期激活增加。与对照组相比，LC 患者中 CD4 和 CD8 T 淋巴细胞表达 CD69 抗原的比例显著更高。与无 VCA IgM 抗体的患者相比，抗-EBNA1 IgG 和抗-VCA IgM 阳性的 LC 患者中 CD4 CD25 T 淋巴细胞的比例较低。
喉癌患者免疫反应功能障碍可能与 EBV 感染有关。
