Dvorožňáková Emília, Bucková Barbora, Hurníková Zuzana, Revajová Viera, Lauková Andrea
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia.
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 04001 Košice, Slovakia.
Vet Parasitol. 2016 Nov 15;231:69-76. doi: 10.1016/j.vetpar.2016.07.004. Epub 2016 Jul 7.
This study focusses on the effect of probiotic (bacteriocinogenic) strains on parasite infection and innate immunity - phagocytosis and oxidative burst of blood monocytes and polymorphonuclear leukocytes (PMNL) in mice infected with Trichinella spiralis. Bacteriocinogenic and probiotic strains of different origin (Enterococcus faecium AL41=CCM8558, Enterococcus durans ED26E/7, Lactobacillus fermentum AD1=CCM7421, Lactobacillus plantarum 17L/1) were administered daily in dose of 10CFU/ml in 100μl and mice were infected with 400 larvae of T. spiralis on 7th day of treatment. Phagocytic activity of blood leukocytes was inhibited at week 3 and 4 post infection (p.i.), i.e. in the time of massive muscle invasion with larvae T. spiralis. Administration of bacterial strains to mice prior to T. spiralis infection elevated and prolonged phagocytic activity of blood leukocytes and their ingestion capability from week 1 to 3 of the infection and the phagocytosis was inhibited only at week 4 p.i. The highest stimulative effect on phagocytosis was induced by strains E. durans ED26E/7, L. fermentum AD1=CCM7421, and L. plantarum 17L/1. The percentage of cells with respiratory burst and their enzymatic activity was increased after T. spiralis infection with the exception of week 3 p.i. In contrast, in all mice treated with bacterial strains the enzymatic stimulation was observed after the infection, with the highest intensity caused by strains E. durans ED26E/7, L. fermentum AD1=CCM7421 and L. plantarum 17L/1. The administration of probiotic strains stimulated phagocytosis and respiratory burst of blood PMNL that could contribute to a decreased larval migration and a destruction of muscle larvae and then reduced parasite burden in the host. The protective effect against T. spiralis infection was induced by all strains, but the highest reduction was recorded by E. faecium AL41=CCM8558.
本研究聚焦于益生菌（产细菌素）菌株对感染旋毛虫的小鼠体内寄生虫感染以及先天免疫——血液单核细胞和多形核白细胞（PMNL）的吞噬作用和氧化爆发的影响。不同来源的产细菌素和益生菌菌株（粪肠球菌AL41 = CCM8558、耐久肠球菌ED26E/7、发酵乳杆菌AD1 = CCM7421、植物乳杆菌17L/1）以10CFU/ml的剂量每日100μl给药，在治疗第7天，小鼠感染400条旋毛虫幼虫。感染后第3周和第4周，即旋毛虫幼虫大量侵入肌肉时，血液白细胞的吞噬活性受到抑制。在旋毛虫感染前给小鼠施用细菌菌株，可提高并延长感染第1周和第3周血液白细胞的吞噬活性及其摄取能力，仅在感染后第4周吞噬作用受到抑制。耐久肠球菌ED26E/7、发酵乳杆菌AD1 = CCM7421和植物乳杆菌17L/1菌株对吞噬作用的刺激效果最强。除感染后第3周外，旋毛虫感染后呼吸爆发细胞的百分比及其酶活性增加。相反，在所有用细菌菌株处理的小鼠中，感染后均观察到酶刺激，其中耐久肠球菌ED26E/7、发酵乳杆菌AD1 = CCM7421和植物乳杆菌17L/1菌株引起的刺激强度最高。施用益生菌菌株刺激了血液PMNL的吞噬作用和呼吸爆发，这可能有助于减少幼虫迁移和破坏肌肉幼虫，进而减轻宿主体内寄生虫负担。所有菌株均对旋毛虫感染产生保护作用，但粪肠球菌AL41 = CCM8558的保护效果最佳。
