Deng Lei, Yue Chan-Juan, Chai Yi-Jun, Wang Wu-You, Su Xiao-Yan, Zhou Zi-Yao, Wang Long-Qiong, Li Ling-Yu, Liu Hai-Feng, Zhong Zhi-Jun, Cao Sui-Zhong, Hu Yan-Chun, Fu Hua-Lin, Peng Guang-Neng
The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, Sichuan Province, 611130, China.
Int J Parasitol Parasites Wildl. 2019 Aug 9;10:164-169. doi: 10.1016/j.ijppaw.2019.08.001. eCollection 2019 Dec.
, a unicellular enteric microsporidian parasite, can infect humans and a wide range of animals throughout the world. Although has been identified in many animals, there is no information regarding the genotypes of in pet birds in China. Birds are important sources of emerging infectious diseases that affect humans, and immunosuppressed individuals can be exposed to potential zoonotic agents shed by birds. The aim of the present study was to determine the prevalence and genotypic diversity of in pet birds, as well as assessed its zoonotic potential. A total of 387 fecal samples were collected from Psittaciformes (n = 295), Passeriformes (n = 67), and Galliformes (n = 16) from four pet markets in Sichuan province, Southwestern China. The overall prevalence of in pet birds was 25.1% based on nested polymerase chain reaction analysis of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene (Psittaciformes, 21.7%; Passeriformes, 37.3%; Galliformes, 50.0%). Eight genotypes of were identified, including five known genotypes (D, SC02, BEB6, CHB1, and MJ5) and three novel genotypes (SCB-I, SCB-II, and SCB-III). In phylogenetic analysis, genotypes D and SC02 and one novel genotype SCB-II were clustered within group 1, genotype BEB6 was classified within group 2, and the remaining genotypes (CHB1, MJ5, SCB-I, and SCB-III) clustered with group 10. To the best of our knowledge, this is the first report of infection in pet birds in China. Genotypes D, SC02, and BEB6 that have been previously identified in humans, were found in pet birds in this study, suggesting that these pet birds can be a potential source of human microsporidiosis in China.
[某种单细胞肠道微孢子虫寄生虫名称]可感染全球范围内的人类和多种动物。尽管已在许多动物中发现了[该寄生虫名称]，但在中国宠物鸟中关于其基因型的信息却尚无报道。鸟类是影响人类的新发传染病的重要来源，免疫抑制个体可能会接触到鸟类排出的潜在人畜共患病原体。本研究的目的是确定宠物鸟中[该寄生虫名称]的感染率和基因型多样性，并评估其人畜共患病潜力。从中国西南部四川省的四个宠物市场收集了总共387份粪便样本，其中鹦鹉目（n = 295）、雀形目（n = 67）和鸡形目（n = 16）。基于核糖体RNA（rRNA）基因内部转录间隔区（ITS）区域的巢式聚合酶链反应分析，宠物鸟中[该寄生虫名称]的总体感染率为25.1%（鹦鹉目，21.7%；雀形目，37.3%；鸡形目，50.0%）。鉴定出了[该寄生虫名称]的八种基因型，包括五种已知基因型（D、SC02、BEB6、CHB1和MJ5）和三种新基因型（SCB - I, SCB - II和SCB - III）。在系统发育分析中，基因型D和SC02以及一种新基因型SCB - II聚集在第1组内，基因型BEB6归类于第2组，其余基因型（CHB1、MJ5、SCB - I和SCB - III）与第10组聚集在一起。据我们所知，这是中国宠物鸟中[该寄生虫名称]感染的首次报道。本研究在宠物鸟中发现了先前在人类中已鉴定出的基因型D、SC02和BEB6，这表明这些宠物鸟可能是中国人类微孢子虫病的潜在来源。
