Song Chunlan, Liu Kun, Wang Zhongjie, Ding Bo, Wang Shengchun, Weng Yue, Chiang Chien-Wei, Lei Aiwen
College of Chemistry and Molecular Sciences , Institute for Advanced Studies (IAS) , Wuhan University , Wuhan 430072 , P. R. China . Email:
National Synchrotron Radiation Research Center (NSRRC) , Hsinchu Science Park , Hsinchu , Taiwan.
Chem Sci. 2019 Jul 8;10(34):7982-7987. doi: 10.1039/c9sc02218j. eCollection 2019 Sep 14.
Directly introducing a beneficial functional group into biomolecules under mild, clean and easy-to-handle conditions is of great importance in the field of chemical biology and pharmacology. Herein, we described an electrochemical strategy to perform the bioconjugation of tyrosine residues with phenothiazine derivatives in a rapid and simple manner. In this electrochemical system, various polypeptides and proteins were successfully labelled with excellent site- and chemo-selectivity, and metals, oxidants or additives were also avoided.
在温和、清洁且易于操作的条件下直接将有益的官能团引入生物分子在化学生物学和药理学领域具有重要意义。在此，我们描述了一种电化学策略，以快速简便的方式实现酪氨酸残基与吩噻嗪衍生物的生物共轭。在这个电化学体系中，各种多肽和蛋白质成功地以优异的位点和化学选择性进行了标记，并且无需使用金属、氧化剂或添加剂。
