Hunt Benjamin James, Mallon Eamonn B, Rosato Ezio
Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
Front Physiol. 2019 Oct 18;10:1325. doi: 10.3389/fphys.2019.01325. eCollection 2019.
The amphipod is a model organism of growing importance in the fields of evolutionary development and regeneration. A small, hardy marine crustacean that breeds year-round with a short generation time, it has simple lab culture requirements and an extensive molecular toolkit including the ability to generate targeted genetic mutant lines. Here we identify canonical core and regulatory clock genes using genomic and transcriptomic resources as a first step in establishing this species as a model in the field of chronobiology. The molecular clock of lacks orthologs of the canonical circadian genes and , in common with the mammalian system but in contrast to many arthropods including . Furthermore the predicted CLOCK peptide is atypical and CRY2 shows an extended 5' region of unknown function. These results appear to be shared by two other amphipod species.
在进化发育和再生领域，端足目动物作为一种模式生物，其重要性日益凸显。它是一种小型、耐寒的海洋甲壳类动物，全年繁殖，世代周期短，对实验室培养条件要求简单，拥有丰富的分子工具，包括能够产生靶向基因突变系。在此，我们利用基因组和转录组资源鉴定了典型的核心时钟基因和调控时钟基因，这是将该物种确立为生物钟学领域模式生物的第一步。与哺乳动物系统相同，但与包括[具体物种]在内的许多节肢动物不同，[端足目动物名称]的分子时钟缺乏典型昼夜节律基因[具体基因1]和[具体基因2]的直系同源基因。此外，预测的CLOCK肽是非典型的，CRY2显示出一个功能未知的延长的5'区域。另外两种端足目物种似乎也有这些结果。
