长链非编码RNA LINC00665通过调控miR-149-3p/RNF2轴促进胃癌肿瘤发生。

Long non-coding RNA LINC00665 gastric cancer tumorigenesis by regulation miR-149-3p/RNF2 axis.

作者信息

Qi Hongyang, Xiao Zhanyu, Wang Yunxi

机构信息

Department of Gastroenterology, The Central Hospital of Xinxiang, Xinxiang, Henan 453000, People's Republic of China.

出版信息

Onco Targets Ther. 2019 Aug 28;12:6981-6990. doi: 10.2147/OTT.S214588. eCollection 2019.

BACKGROUND

Recently, LINC00665 has been reported to be a pivotal regulator in kinds of malignancy, such as lung cancer and liver cancer. However, the functions and underlying mechanisms of LINC00665 in gastric cancer (GC) remain unclear.

MATERIALS AND METHODS

We recruited 49 paired GC tissue to explore LINC00665 expression by qRT-PCR. In vitro function assays were used to explore the roles of LINC00665 in GC progression. Moreover, the interaction among LINC00665, miR-149-3p and RNF2 was explored by bioinformatics analysis and luciferase reporter assay.

RESULTS

In the present study, we found that LINC00665 expression was significantly elevated in GC tissues and cell lines. High LINC00665 expression was associated with TNM stage, histological grade, and poor prognosis of GC patients. Function assays showed that LINC00665 suppression significantly reduced GC cells viability and invasion ability in vitro. Mechanistic analysis showed that LINC00665 might serve as a ceRNA for miR-149-3p to regulate the expression of RNF2.

CONCLUSION

Our current study revealed the LINC00665/miR-149-3p/RNF2 axis was involved in GC progression, providing novel insights into the treatment for GC.

背景

最近，有报道称LINC00665在肺癌和肝癌等多种恶性肿瘤中是关键调节因子。然而，LINC00665在胃癌（GC）中的功能及潜在机制仍不清楚。

材料与方法

我们收集了49对GC组织，通过qRT-PCR检测LINC00665的表达。采用体外功能试验探究LINC00665在GC进展中的作用。此外，通过生物信息学分析和荧光素酶报告基因检测探究LINC00665、miR-149-3p和RNF2之间的相互作用。

结果

在本研究中，我们发现LINC00665在GC组织和细胞系中的表达显著升高。LINC00665高表达与GC患者的TNM分期、组织学分级及预后不良相关。功能试验表明，抑制LINC00665可显著降低GC细胞的体外活力和侵袭能力。机制分析表明，LINC00665可能作为miR-149-3p的竞争性内源RNA（ceRNA）来调节RNF2的表达。