Qi Ya-Lin, Chen Jian, Zhang Bo, Li Hua, Li Dong-Dong, Wang Bao-Zhong, Yang Yu-Shun, Zhu Hai-Liang
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
College of Chemical Engineering, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 15;227:117707. doi: 10.1016/j.saa.2019.117707. Epub 2019 Oct 30.
In this work, a primary method was constructed for detecting hydrazine in plant, thus accomplished the closed-loop monitoring of hydrazine circulation within manufacture, environment, plants, animals and human. From a series of sensors, QYL-1 was selected to present the hydrazine sensing properties. As a preliminary tool, QYL-1 suggested the ultra-wide linear range (0-20.0 equivalent) and high selectivity, which were extremely essential for linking the monitoring in various scale and field. For the first time, concentration-dependent tracking of hydrazine was successfully performed in Arabidopsis Thaliana root tips. Afterwards applications in water samples and living MCF-7 cells then fulfilled the demonstration of closing the loop by linking both the upstream and downstream nodes. More than raising a practical method, this work offered initial information for the closed-loop monitoring of hydrazine circulation, which might be significant for the ideal systematic managing in future.
在这项工作中，构建了一种用于检测植物中肼的主要方法，从而实现了对肼在制造、环境、植物、动物和人类之间循环的闭环监测。从一系列传感器中，选择了QYL-1来展示其对肼的传感特性。作为一种初步工具，QYL-1显示出超宽线性范围（0-20.0当量）和高选择性，这对于连接各种规模和领域的监测极为重要。首次在拟南芥根尖中成功进行了肼的浓度依赖性追踪。随后在水样和活的MCF-7细胞中的应用通过连接上下游节点完成了闭环演示。这项工作不仅提出了一种实用方法，还为肼循环的闭环监测提供了初步信息，这可能对未来理想的系统管理具有重要意义。
