Departamento de Ciencias Básicas Biomédicas, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, Chile.
Laboratorio de Cultivo Celular, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, Chile.
Molecules. 2019 Nov 7;24(22):4028. doi: 10.3390/molecules24224028.
The fruits from the Chilean Podocarpaceae have been consumed since pre-Hispanic times. Little is known about the composition and biological properties of this fruit. The aim of this work was to identify the secondary metabolites of the edible part of fruits and to assess their antioxidant activity by means of chemical and cell-based assays. Methanol extracts from fruits were fractionated on a XAD7 resin and the main compounds were isolated by chromatographic means. Antioxidant activity was determined by means of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing power (FRAP), trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays. The cytoprotective activity of the extract against oxidative and dicarbonyl stress was evaluated in human gastric epithelial cells (AGS). The total intracellular antioxidant activity (TAA) of the extract was determined in AGS cells. The inhibition of meat lipoperoxidation was evaluated under simulated gastric digestion conditions. Rutin, caffeic acid β-glucoside and 20-hydroxyecdysone were identified as major components of the fruit extract. Additional compounds were identified by high-performance liquid chromatography diode-array detector mass spectrometry (HPLC-DAD-MS) and/or co-injection with standards. Extracts showed dose-dependent cytoprotective effects against oxidative and dicarbonyl-induced damage in AGS cells. The TAA increased with the pre-incubation of AGS cells with the extract. This is the first report on the composition and biological activity of this Andean fruit.
智利南洋杉科的果实自前西班牙时期就已被食用。然而，对于这种果实的组成成分和生物特性，人们知之甚少。本研究旨在鉴定可食用部分果实中的次生代谢产物，并通过化学和基于细胞的测定方法评估其抗氧化活性。采用 XAD7 树脂对果实的甲醇提取物进行了分级，并用色谱方法分离出主要化合物。通过 2,2-二苯基-1-苦基肼基（DPPH）自由基、铁还原能力（FRAP）、trolox 等效抗氧化能力（TEAC）和氧自由基吸收能力（ORAC）测定法测定了抗氧化活性。通过评估提取物对人胃上皮细胞（AGS）氧化应激和二羰基应激的细胞保护活性，研究了提取物的总细胞内抗氧化活性（TAA）。在 AGS 细胞中测定了提取物的抑制肉脂过氧化活性。鉴定出芦丁、咖啡酸-β-葡萄糖苷和 20-羟基蜕皮甾酮为果实提取物的主要成分。通过高效液相色谱二极管阵列检测器质谱（HPLC-DAD-MS）和/或与标准品共注射，进一步鉴定了其他化合物。提取物对 AGS 细胞的氧化和二羰基诱导损伤具有剂量依赖性的细胞保护作用。AGS 细胞与提取物预孵育后，TAA 增加。这是首次对这种安第斯水果的组成和生物活性进行的报告。
