Parham D M, Slidders W, Robertson A J
Department of Pathology, University of Dundee, Ninewells Hospital and Medical School, Scotland.
J Clin Pathol. 1988 Aug;41(8):875-9. doi: 10.1136/jcp.41.8.875.
Expression of human milk fat globule (HMFG1) in immunohistochemically stained sections of breast carcinomas was assessed subjectively and objectively from 82 women (age range 41-96 years) to determine its prognostic importance. No correlation was observed between the degree of staining and prognosis even when the subcellular distribution of antigen expression was assessed. The total absence of staining with HMFG1 was possibly associated with a favourable outcome, although this did not quite achieve significance with the small numbers involved. The Quantimet 970 was used for objective semiautomated measurement of immunohistochemical reactions in paraffin wax sections and was found to produce better resolution and to eliminate subjective error.
通过主观和客观评估82名女性（年龄范围41 - 96岁）乳腺癌免疫组织化学染色切片中人类乳脂肪球（HMFG1）的表达，以确定其预后重要性。即使评估抗原表达的亚细胞分布，也未观察到染色程度与预后之间的相关性。HMFG1染色完全缺失可能与良好预后相关，尽管由于样本数量少，这一结果未达到统计学显著性。使用Quantimet 970对石蜡切片中的免疫组织化学反应进行客观半自动测量，发现其具有更好的分辨率并消除了主观误差。
