Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland.
Colloids Surf B Biointerfaces. 2020 Jan 1;185:110605. doi: 10.1016/j.colsurfb.2019.110605. Epub 2019 Oct 24.
Polymeric coatings with positive surface charge offer potential antimicrobial activity, which they owe to a simple electrostatic attraction with negatively charged bacterial walls and membranes. We describe synthesis and characterization of poly(2-aminoethyl methacrylate) and its copolymers with methyl methacrylate and butyl acrylate, as potential binders for antimicrobial solvent-cast paints. TiO and CaCO mineral particles were employed as model pigments/fillers, as they are used in most real-life paint formulations. Electrokinetic (ζ) potential and antimicrobial activity of thin films made of the (co)polymers in the absence and presence of TiO and CaCO nanopowders were assessed using streaming current measurements and microbial growth inhibition tests, respectively. Independently of the structure of the monomers used for the synthesis, the films showed positive ζ-potential values (up to +95 mV) in the pH range 3.5-8.0. The presence of mineral particles at 50% dry weight of the films did not affect significantly the ζ(pH) curves. The films made of the mixed dispersions remained positively charged and inhibited growth of both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria, as well as yeast (C. albicans). The mixed polymeric-mineral films described in this study seem to be promising potential candidates for designing antimicrobial coatings aimed to prevent spreading of bacterial infections.
带正表面电荷的聚合涂层具有潜在的抗菌活性，这归因于它们与带负电荷的细菌细胞壁和膜之间的简单静电吸引。我们描述了聚（2-氨基乙基甲基丙烯酸酯）及其与甲基丙烯酸甲酯和丙烯酸丁酯的共聚物的合成和表征，作为抗菌溶剂铸造涂料的潜在粘合剂。TiO 和 CaCO 矿物颗粒被用作模型颜料/填料，因为它们被用于大多数实际涂料配方中。使用流动电流测量和微生物生长抑制测试分别评估了（共）聚合物在不存在和存在 TiO 和 CaCO 纳米粉末的情况下制成的薄膜的电动（ζ）电位和抗菌活性。独立于用于合成的单体的结构，在 pH 值 3.5-8.0 范围内，薄膜显示出正 ζ-电位值（高达+95 mV）。在 50%干重的薄膜中存在矿物颗粒时，ζ（pH）曲线没有明显影响。由混合分散体制成的薄膜仍然带正电，并抑制革兰氏阴性（大肠杆菌）和革兰氏阳性（金黄色葡萄球菌）细菌以及酵母（白色念珠菌）的生长。本研究中描述的混合聚合物-矿物薄膜似乎是设计抗菌涂层的有前途的潜在候选材料，旨在防止细菌感染的传播。
