一种独立的生物反应器系统，可在灌注流动下向负载 hBMSC 的 3D 壳聚糖-石墨烯模板施加压缩载荷。

A standalone bioreactor system to deliver compressive load under perfusion flow to hBMSC-seeded 3D chitosan-graphene templates.

机构信息

Institute of Biomedical and Neural Engineering, Reykjavík University, Menntavegur 1, 101, Reykiavík, Iceland.

Laboratory of Cellular and Molecular Engineering "Silvio Cavalcanti" - Department of Electrical, Electronic and Information Engineering "Guglielmo Marconi" (DEI), University of Bologna, Via Cesare Pavese 50, 47522, Cesena, FC, Italy.

出版信息

Sci Rep. 2019 Nov 14;9(1):16854. doi: 10.1038/s41598-019-53319-7.

The availability of engineered biological tissues holds great potential for both clinical applications and basic research in a life science laboratory. A prototype standalone perfusion/compression bioreactor system was proposed to address the osteogenic commitment of stem cells seeded onboard of 3D chitosan-graphene (CHT/G) templates. Testing involved the coordinated administration of a 1 mL/min medium flow rate together with dynamic compression (1% strain at 1 Hz; applied twice daily for 30 min) for one week. When compared to traditional static culture conditions, the application of perfusion and compression stimuli to human bone marrow stem cells using the 3D CHT/G template scaffold induced a sizable effect. After using the dynamic culture protocol, there was evidence of a larger number of viable cells within the inner core of the scaffold and of enhanced extracellular matrix mineralization. These observations show that our novel device would be suitable for addressing and investigating the osteogenic phenotype commitment of stem cells, for both potential clinical applications and basic research.

工程化生物组织的可用性在临床应用和生命科学实验室的基础研究中都具有巨大的潜力。提出了一种原型独立式灌注/压缩生物反应器系统，以解决接种在 3D 壳聚糖-石墨烯（CHT/G）模板上的干细胞的成骨定向。测试涉及协调管理 1ml/min 的介质流速和动态压缩（1Hz 时 1%应变；每天两次，每次 30 分钟），持续一周。与传统的静态培养条件相比，使用 3D CHT/G 模板支架对人骨髓干细胞进行灌注和压缩刺激产生了显著的效果。在使用动态培养方案后，支架内部核心区域内有更多存活细胞的证据，并且细胞外基质矿化增强。这些观察结果表明，我们的新型设备将适用于解决和研究干细胞的成骨表型定向，无论是潜在的临床应用还是基础研究。