Toledano Manuel, Toledano-Osorio Manuel, Navarro-Hortal María D, Varela-López Alfonso, Osorio Raquel, Quiles José L
Faculty of Dentistry, Dental Materials Section, University of Granada, Colegio Máximo de Cartuja s/n, 18071 Granada, Spain.
Department of Physiology, Institute of Nutrition and Food Technology "José Mataix Verdú", Biomedical Research Center, University of Granada, Avda del Conocimiento s/n., 18100 Granada, Spain.
Antioxidants (Basel). 2019 Nov 14;8(11):550. doi: 10.3390/antiox8110550.
The objective was to evaluate the toxicity of zinc- and doxycycline-loaded polymeric nanoparticles (NPs) using as a model organism. These NPs are composed of ethylene glycol dimethacrylate, 2-hydroxyethyl methacrylate and methacrylic acid. NPs were loaded with doxycycline (D-NPs) and zinc (Zn-NPs) by chemical adsorption, and loading efficacy was demonstrated. Worm death rate in a concentration-response curve basis was calculated for lethality. Metabolism was evaluated through pharyngeal pumping assay. Body length measurements, brood size and egg lays were used to gauge growth, reproduction and fertility respectively. Intracellular hydrogen peroxide levels were determined to assess the reactive oxygen species production. One-way ANOVA and Bonferroni were used for comparisons ( < 0.05). Tested NPs at the highest dosage did not affect lethality or worm metabolism, expressed in terms of death rate and pharyngeal pumping per minute, respectively. Zn-NPs slightly increased worm growth. The concentration of the intracellular hydrogen peroxide levels was the lowest in the D-NPs group. The distinct NPs and concentrations employed were shown to be non-toxic for in situ administration of zinc and doxycycline, reducing the harmful effects of these compounds.
目的是使用作为模式生物来评估负载锌和多西环素的聚合物纳米颗粒（NPs）的毒性。这些纳米颗粒由乙二醇二甲基丙烯酸酯、甲基丙烯酸2-羟乙酯和甲基丙烯酸组成。通过化学吸附使纳米颗粒负载多西环素（D-NPs）和锌（Zn-NPs），并证明了负载效率。以浓度-反应曲线为基础计算蠕虫死亡率以评估致死率。通过咽泵试验评估新陈代谢。分别使用体长测量、产卵量和产蛋数来衡量生长、繁殖和生育能力。测定细胞内过氧化氢水平以评估活性氧的产生。使用单向方差分析和Bonferroni法进行比较（<0.05）。最高剂量的受试纳米颗粒分别以死亡率和每分钟咽泵次数表示，对致死率或蠕虫新陈代谢没有影响。Zn-NPs略微增加了蠕虫的生长。D-NPs组细胞内过氧化氢水平的浓度最低。所使用的不同纳米颗粒和浓度对锌和多西环素的原位给药无毒，减少了这些化合物的有害影响。
