Department of Medicinal Chemistry, Theodor Bilharz Research Institute, Kornaish El-Nile St, Giza, 12411, Egypt.
Department of Biochemistry and Molecular Biology, Theodor Bilharz Research Institute, Kornaish El-Nile St, Giza, 12411, Egypt.
BMC Complement Med Ther. 2024 Mar 14;24(1):122. doi: 10.1186/s12906-024-04420-6.
The Convolvulus genus is distributed all over the world and has a long history in traditional medicine. As nanotechnology expands its reach into areas like drug delivery and biomedicine, this study intends to assess the potential of Convolvulus arvensis L. extracts as anti-bacterial, anti-inflammatory and anti-cancer agents, along with chemical profiling of the methanolic (MeOH) extract active ingredients.
The chemical composition of an 85% MeOH extract was investigated by liquid chromatography with an electrospray source connected to mass spectrometry (LC-ESI-MS). Both the 85% MeOH extract and n-butanol fraction of C. arvensis were loaded for the first time on alginate/chitosan nanoparticles. The 85% MeOH extract, n-butanol fraction and their loaded nanoparticles were tested for their cytotoxicity, anticancer, anti-inflammatory and antibacterial activity (against pathogenic bacteria, E. coli and S. aureus).
The chemical investigation of 85% MeOH extract of C. arvensis underwent LC-ESI-MS analysis, revealing twenty-six phenolic substances, of which 16 were phenolic acids, 6 were flavonoids, 1 glycolipid, 1 sesquiterpene and 2 unknown compounds. The FT-IR spectra confirmed the encapsulation of the 85% MeOH extract and n-butanol fraction onto alginate/chitosan nanoparticles and small size obtained by TEM maintained them nontoxic and enhanced their anti-inflammatory activity (the IC was decreased from 1050 to 175 µg/ml). The anti-cancer activity against HepG2 was increased and the cell viability was decreased from 28.59 ± 0.52 to 20.80 ± 0.27 at a maximum concentration of 1000 µg/ml. In addition, the MIC of encapsulated extracts was decreased from 31.25 to7.78 µg/ml in E. coli (Gm-ve) and from 15.56 to 7.78 µg/ml in S. aureus (Gm + ve) bacteria.
Both alginate and chitosan are excellent natural polymers for the encapsulation process, which affects positively on the bioactive constituents of C. arvensis extracts and improves their biological properties.
旋花属植物分布于世界各地，在传统医学中有着悠久的历史。随着纳米技术在药物输送和生物医学等领域的应用不断扩大，本研究旨在评估旋花属植物提取物作为抗菌、抗炎和抗癌药物的潜力，并对其甲醇（MeOH）提取物的活性成分进行化学分析。
采用液相色谱-电喷雾源-质谱联用技术（LC-ESI-MS）对 85% MeOH 提取物的化学成分进行了研究。首次将 85% MeOH 提取物和旋花属植物的正丁醇部分加载到海藻酸钠/壳聚糖纳米粒子上。对 85% MeOH 提取物、正丁醇部分及其负载的纳米粒子进行了细胞毒性、抗癌、抗炎和抗菌活性（针对致病菌、大肠杆菌和金黄色葡萄球菌）的测试。
对旋花属植物 85% MeOH 提取物进行了 LC-ESI-MS 分析，揭示了 26 种酚类物质，其中 16 种为酚酸，6 种为黄酮类化合物，1 种为糖脂，1 种为倍半萜类化合物，2 种为未知化合物。FT-IR 光谱证实了 85% MeOH 提取物和正丁醇部分被包封到海藻酸钠/壳聚糖纳米粒子上，并且 TEM 获得的小尺寸使其保持非毒性，并增强了其抗炎活性（IC 从 1050 降至 175μg/ml）。对 HepG2 的抗癌活性增强，细胞活力从 1000μg/ml 时的 28.59±0.52%降至 20.80±0.27%。此外，包封提取物的 MIC 从大肠杆菌（Gm-ve）中的 31.25 降至 7.78μg/ml，从金黄色葡萄球菌（Gm+ve）中的 15.56 降至 7.78μg/ml。
海藻酸钠和壳聚糖都是包封过程的优良天然聚合物，这对旋花属植物提取物的生物活性成分产生了积极的影响，并提高了它们的生物学特性。
