Department of Pharmaceutics and Bioengineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
College of Chemistry and Environment Protection Engineering, Southwest Minzu University, Chengdu 610041, PR China.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110185. doi: 10.1016/j.msec.2019.110185. Epub 2019 Sep 10.
Silver nanoparticles (AgNPs) are effective antimicrobial reagent, especially for the treatment of multidrug-resistant microorganisms. However, it is difficult to remove the residual harmful reducing agents in AgNPs synthesized by the traditional chemical reduction method. In addition, AgNPs exhibit cytotoxicity when exposed directly to cells for biomedical applications and will rapidly aggregate in aqueous environment. Herein, we develop a new route to synthesize submicron-particles containing AgNPs (κC@Ag MPs) with high aqueous stability, robust antibacterial activity and low cytotoxicity. AgNPs were firstly greenly synthesized using κ-carrageenan as environmental friendly reducing and stabilizing agent under heating in aqueous solution. Then the AgNPs were immobilized in the net of κ-carrageenan by adding KCl to obtain κC@Ag MPs. The results indicated that κC@Ag MPs were quite stable without any agglomeration and precipitation in aqueous solution for more than three months. κC@Ag MPs exhibited robust antibacterial activity against Gram-positive and Gram-negative bacteria, even better than that of pure AgNPs. Notably, κC@Ag MPs maintained the long-lasting retention of antibacterial activity. In addition, κC@Ag MPs possessed well cytocompatibility towards the L02 and L929 cells. Overall, κC@Ag MPs may have prospective application as effective and sustainable antibacterial agent in biomedical fields.
银纳米粒子（AgNPs）是一种有效的抗菌试剂，特别是对治疗多药耐药微生物非常有效。然而，用传统的化学还原法合成的 AgNPs 中，很难去除残留的有害还原剂。此外，AgNPs 在用于生物医学应用时直接暴露于细胞中会表现出细胞毒性，并且在水相环境中会迅速聚集。在此，我们开发了一种新的方法来合成具有高水分散稳定性、强抗菌活性和低细胞毒性的含 AgNPs 的亚微米颗粒（κC@Ag MPs）。AgNPs 首先在水溶液中通过加热，使用κ-卡拉胶作为环保还原剂和稳定剂进行绿色合成。然后，通过添加 KCl 将 AgNPs 固定在κ-卡拉胶的网络中，得到 κC@Ag MPs。结果表明，κC@Ag MPs 在水溶液中非常稳定，超过三个月没有任何聚集和沉淀。κC@Ag MPs 对革兰氏阳性菌和革兰氏阴性菌均表现出强大的抗菌活性，甚至优于纯 AgNPs。值得注意的是，κC@Ag MPs 保持了持久的抗菌活性。此外，κC@Ag MPs 对 L02 和 L929 细胞具有良好的细胞相容性。总体而言，κC@Ag MPs 可能作为生物医学领域中有效且可持续的抗菌剂具有广阔的应用前景。
