Ceramic Physics Laboratory, Kyoto Institute of Technology, Sakyo-ku, Matsugasaki, 606-8585, Kyoto, Japan; Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, 602-8566, Japan.
Department of Dental Medicine, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto, 602-8566, Japan.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110278. doi: 10.1016/j.msec.2019.110278. Epub 2019 Oct 7.
Using a simple and innovative sandblasting process, disks of monolithic biomedical silicon nitride (β-SiN) were texturized with a matrix of regular, discrete square trenches with a total depth in the range of hundreds of microns. The process consisted of sandblasting SiN substrates through a stainless-steel wire-mesh (150 or 200 μm) using abrasive silicon carbide powders (α-SiC, ∼40 μm) under 1,034 kPa (150 psi) of gas pressure. The depth of the porosities could be controlled varying both the treatment time and the distance from the surface. Part of the samples were then filled with 45S5 Bioglass® powders to improve the osteointegration and stimulate the production of bone tissue. Due to the increased macroscopic and microscopic roughness, biological testing using human osteosarcoma cells (SaOS-2) showed improved cell proliferation and greater production of both mineral (hydroxyapatite) and organic (collagen) phases on the patterned surfaces compared to untreated β-SiN or to the biomedical titanium control samples. Both of these effects were further enhanced when the porosities were filled with Bioglass®.
采用简单而创新的喷砂工艺，使用具有规则离散方形凹槽矩阵的生物医学整块氮化硅（β-SiN）圆盘进行纹理处理，凹槽总深度在数百微米范围内。该工艺通过在 1034 kPa（150 psi）的气压下使用碳化硅磨料（α-SiC，约 40 µm）通过不锈钢丝筛（150 或 200 µm）对 SiN 基底进行喷砂处理。通过改变处理时间和表面距离可以控制孔隙的深度。部分样品随后用 45S5 Bioglass®粉末填充，以提高骨整合并刺激骨组织的产生。由于宏观和微观粗糙度的增加，使用人骨肉瘤细胞（SaOS-2）进行的生物测试表明，与未处理的β-SiN或生物医学钛对照样品相比，图案化表面上的细胞增殖和矿物质（羟基磷灰石）和有机（胶原）相的产生都得到了改善。当用 Bioglass®填充孔隙时，这两种效果都得到了进一步增强。
